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Key Documents

P3288

Sigma-Aldrich

Phosphate buffer pH 7.2 at 25 °C

for hematology and histology staining techniques

Synonym(s):

phosphate buffer

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About This Item

UNSPSC Code:
12352107
NACRES:
NA.47

Quality Level

200

form

powder

color

white

pH

7.10-7.30

application(s)

hematology
histology

storage temp.

room temp

Related Categories

General description

Phosphate buffer is the most commonly used component of irrigation solution during surgeries, to preserve the living tissues. It is also used in immunochemical staining procedures. Phosphate buffer serves as an important fixative for light and electron microscopy. Its buffering capacity is pH sensitive and is preferred to be used at physiological pH (7.2-7.4).

Application

Intended for use as a buffer in various Romanowsky type staining procedures, including Wright Stain, Wright Giemsa, Giemsa, May Grunwald, Jenner and Leishman. Phosphate buffer pH 7.2 at 25 °C has been used in oocyte collection from porcine ovaries.

Reconstitution

Each vial will prepare 3.8 L of buffer, 8.3 mM.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000376999
0000325234
0000311282
0000299192
0000325234

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  5. How to solubilize Product P3288, Phosphate Buffer pH 7.2?

    The instructions for reconstitution of Product P3288, Phosphate buffer pH 7.2 at 25 °C are indicated on the Wright-Giemsa protocol (see attached packaging insert).Briefly, Product P3288, Phosphate buffer pH 7.2 should be prepared by diluting 1 vial of buffer to 1 gallon or 3.8 liters of deionized water. Mix well to dissolve. Store Phosphate Buffer at room temperature (18-26°C). Store working phosphate buffer solution at 2-8°C. Discard the working phosphate solution if turbidity or visible bacterial growth is present.

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Low concentrations of MEM vitamins during in vitro maturation of porcine oocytes improves subsequent parthenogenetic development
Naruse K, et al.
Theriogenology, 67(2), 407-412 (2007)
Biological Electron Microscopy: Theory, Techniques, and Troubleshooting (2011)
Thaise L Teixeira et al.
Microbial pathogenesis, 135, 103618-103618 (2019-07-17)
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