P2850
Phosphatase Inhibitor Cocktail 1
DMSO solution, mixture of inhibitors
Synonym(s):
Phosphatase Inhibitor
About This Item
Recommended Products
Product Name
Phosphatase Inhibitor Cocktail 1, DMSO solution
form
DMSO solution
Quality Level
shipped in
wet ice
storage temp.
2-8°C
General description
Application
Biochem/physiol Actions
Quantity
Analysis Note
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
Regulatory Information
Choose from one of the most recent versions:
Certificates of Analysis (COA)
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How stable is Product P2850, Phosphatase Inhibitor Cocktail 1? Can I freeze it?
This reagent, as supplied and stored at 2-8°C, will be stable for four years from the date of manufacturing. Freezing is not recommended, because it may cause precipitation of the inhibitors.
At what concentration should I use Product P2850, Phosphatase Inhibitor Cocktail 1?
In most circumstannces, the product is used at a 100-fold dilution. This will inhibit the phosphatases in 5 mg of protein per mL of lysate.
Product P2850, Phosphatase Inhibitor Cocktail 1, is solid when it arrived. Is it suitable to use?
Since this solution is supplied in DMSO, it may solidify (melting point of DMSO is 16-19 deg C). Simply allow it to melt before use. This will not affect the product.
What inhibitors are present in Product P2850, Phosphatase Inhibitor Cocktail 1, and in what concentrations?
Cantharidin (Product Code C7632) inhibits protein phosphatase 2A (PP-2A). Bromotetramisole (Product Code B8132) inhibits alkaline phosphatases. Microcystin LR (Product Code M2912) inhibits protein phosphatases 1 and 2A (PP-1 and PP-2A). The concentrations of these inhibitors are considered proprietary.
Is Product P2850, Phosphatase Inhibitor Cocktail 1, suitable for use on yeast?
As far as we know, the phosphatases in mammalian cells also appear in yeast cells. Thus both cocktails, P5726 (Phosphatase cocktail II and P2850 (Phosphatase Inhibitor cocktail I), may be needed with yeast extracts.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Articles
The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.
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