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Merck
CN

P2693

Peptide Standards

lyophilized powder

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About This Item

UNSPSC Code:
12352200
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form

lyophilized powder

storage temp.

−20°C

Application

Suitable for use as a capillary zone electrophoresis calibration standard in uncoated capillaries. Using capillary zone electrophoresis, peptides migrate in free solution through a coated or uncoated fused silica capillary with an applied electric field. Proteins are separated by their charge-to-mass ratio. Narrow diameter capillaries (less than 100 μm) allow heat to dissipate efficiently so that high electric fields can be applied to the capillary, leading to high separation efficiencies of peptides. Using peptide standards for determining performance of the capillary is recommended before applying unknown samples. Sigma′s peptide standards have been selected to produce nine well-defined peaks using peptide separation buffer (P2188).

Preparation Note

Reconstitute with 50 μl of peptide separation buffer and 450 μl of deionized water.

Other Notes

Contains approx. 25 μg of each of nine peptides: Bradykinin, Bradykinin fragment 1-5, Substance P, [Arg8]-vasopressin, Luteinizing hormone releasing hormone, Bombesin, Leucine enkephalin, Methionine enkephalin, Oxytocin.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Information

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Zone electrophoresis in open tubular glass capillaries.
Jorgenson, J. and Lukacs, K.D.
Analytical Chemistry, 53, 1298-1298 (1981)
Capillary electrophoresis analysis of recombinant proteins.
G Teshima et al.
Methods in enzymology, 271, 264-293 (1996-01-01)
Kate J Treharne et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 24(5-6), 347-360 (2009-11-17)
Deletion of phenylalanine-508 (DeltaF508) from the first nucleotide-binding domain (NBD1) in the wild-type cystic fibrosis (CF) transmembrane-conductance regulator (wtCFTR) causes CF. However, the mechanistic relationship between DeltaF508-CFTR and the diversity of CF disease is unexplained. The surface location of F508

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