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P1620

Sigma-Aldrich

Anti-PRMT1 antibody, Mouse monoclonal

clone PRMT1-171, purified from hybridoma cell culture

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Synonym(s):
Anti-Protein-Arginine Methyl Transferase 1
MDL number:
MFCD04975120
UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PRMT1-171, monoclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

mouse, chicken, bovine, human, canine, hamster, monkey, rat

concentration

~2 mg/mL

technique(s)

immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 2 μg/mL using whole cell extract of 293T cells

isotype

IgG1

UniProt accession no.

Q99873

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PRMT1(3276)

General description

Protein arginine methyltransferase 1 (PRMT1) gene is localized on human chromosome 19q13.33. It is part of the type I PRMT family.

Specificity

Does not cross-react with PRMT2, 3, 4, and 5.

Immunogen

full length PRMT1. Does not cross-react with PRMT2, 3, 4, and 5.

Application

Anti-PRMT1 antibody, Mouse monoclonal has been used for Immunofluorescence and Western Blotting.

Biochem/physiol Actions

Protein arginine methyltransferase 1 (PRMT1) is crucial for the formation of asymmetric dimethylarginine. It can methylate Twist-1, a transcription factor. The protein also methylates histones and thus modulates transcription and protein activity. PRMT1 is involved in epithelial-mesenchymal transition and may have a role in cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 C for up to one month. For prolonged storage, freeze in working aliquots at 20 C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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PRMT1-mediated arginine methylation controls ATXN2L localization.
Kaehler C, et al.
Experimental Cell Research, 334(1), 114-125 (2015)
PRMT1 promotes glucose toxicity-induced β cell dysfunction by regulating the nucleo-cytoplasmic trafficking of PDX-1 in a FOXO1-dependent manner in INS-1 cells.
Lv L, et al.
Endocrine, 49(3), 669-682 (2015)
PRMT1 Is a Novel Regulator of Epithelial-Mesenchymal-Transition in Non-small Cell Lung Cancer.
Avasarala S, et al.
The Journal of Biological Chemistry, 290(21), 13479-13489 (2015)
Common fusion transcripts identified in colorectal cancer cell lines by high-throughput RNA sequencing.
Nome T, et al.
Translational Oncology, 6(5), 546-IN5-546-IN5 (2013)
Li Liu et al.
Scientific reports, 6, 21810-21810 (2016-02-26)
Protein arginine methyltransferase 1 (PRMT1) catalyzes methylation of histones and other cellular proteins, and thus regulates gene transcription and protein activity. In antigen-induced pulmonary inflammation (AIPI) PRMT1 was up-regulated in the epithelium, while in chronic AIPI, increased PRMT1 shifted to
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