P0089
Anti-Prox1 antibody , Mouse monoclonal
clone 4G10, purified from hybridoma cell culture
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Synonym(s):
Anti-Homeobox prospero-like protein Prox1, Anti-Prospero homeobox 1
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
4G10, monoclonal
form
buffered aqueous solution
mol wt
antigen ~84 kDa
species reactivity
chicken, rat, lizard, human
concentration
~1.5 mg/mL
technique(s)
immunocytochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HepG2 cells
western blot: suitable
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PROX1(5629)
rat ... Prox1(305066)
General description
Monoclonal Anti-Prox1 (mouse IgG2b isotype) is derived from the hybridoma 4G10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant fusion protein of human Prox1. Prospero related homeobox gene 1 (Prox1) belongs to transcription factor subfamily and is homologous to Drosophila homeobox protein, prospero.
Immunogen
recombinant fusion protein corresponding to amino acids 547-737 of human Prox1.
Application
Anti-Prox1 antibody, Mouse monoclonal has been used in various immunochemical techniques.
Biochem/physiol Actions
Prospero related homeobox gene 1 (Prox1) is a key regulator for neuroblast fate determination and photoreceptor development. Prox1 regulates cell differentiation and organogenesis in lens, retina, lymphatic vessels, liver and pancreas. It was found to act as a tumor suppressor gene, because its deficiency or its mutated forms have been implicated in various human cancers. Knockdown of Prox1 by siRNA or by RNA mutations significantly accelerated the in vitro and in vivo growth of hepatocellular carcinoma (HCC) as well as esophageal cancer cells. Prox1 is hypermethylated and transcriptionally silenced in primary and metastatic breast cancer.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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Loss of Function of the Candidate Tumor Suppressor prox1 by RNA Mutation in Human Cancer Cells
Takahashi M, et al.
Neoplasia, 8(12), 1003-1010 (2006)
Xiaoren Chen et al.
Hybridoma (2005), 25(1), 27-33 (2006-02-16)
Prox1 is a divergent homeodomain protein important for the development of the lens, retina, liver, pancreas, and lymphatic vasculature. Prox1 expression is highly upregulated in transformed hepatocytes and has been used as a marker to distinguish lymphatic from blood vasculature.
Molecular mechanisms of lymphatic vascular development
Makinen T, et al.
Cellular and Molecular Life Sciences, 64(15), 1915-1929 (2007)
Epigenetic silencing of the candidate tumor suppressor gene PROX1 in sporadic breast cancer
Versmold B, et al.
International Journal of Cancer. Journal International Du Cancer, 121(3), 547-554 (2007)
Guadalupe Álvarez-Hernán et al.
Experimental eye research, 209, 108681-108681 (2021-06-25)
Planar cell polarity (PCP) is evolutionary conserved and play a critical role in proper tissue development and function. During central nervous system development, PCP proteins exhibit specific patterns of distribution and are indispensable for axonal growth, dendritogenesis, neuronal migration, and
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