P0065
Phospholipase D from Streptomyces chromofuscus
≥50,000 units/mL, buffered aqueous glycerol solution
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form
buffered aqueous glycerol solution
Quality Level
specific activity
≥50,000 units/mL
mol wt
~60 kDa
shipped in
dry ice
storage temp.
−70°C
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General description
Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues.
Application
Phospholipase D from Streptomyces chromofuscus has been used to cleave choline-containing phospholipids (PCs).
Biochem/physiol Actions
Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholines (PC) to yield phosphatidic acid (PA) and choline. PC-PLD activity is regulated by hormones, cytokines, neurotransmitters and growth factors. Thus, it is considered to have a role in signal transduction in various cell types. Three vital lipid messengers produced by PLD plays a vital role in various crucial cellular processes and these lipids also have an essential role in downstream triglyceride and phospholipid synthetic pathway. PLD is essential for exocytosis in neurons and endocrine cells. In addition, it also plays a key role in phagocytosis.
Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Unit Definition
One unit will release 1 μmol of choline from L-α-phosphatidylcholine per hour at pH 8.0 and 30 °C.
Physical form
Solution in 100 mM Tris/HCl, pH 8.0, 10% glycerol (v/v), and 0.1% Triton X-100 (w/v).
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Phospholipase D: a downstream effector of ARF in granulocytes.
Cockcroft S
Science, 263(5146), 523-526 (1994)
Lecithin: a by-product of biodiesel production and a source of choline for dairy cows
Italian Journal of Animal Science, 11:2 (2012)
Eliminating office barriers to the handicapped patient.
Henson JL
The New dentist, 9(6), 30-32 (1979)
Dynamics and function of phospholipase D and phosphatidic acid during phagocytosis.
Traffic, 7(3), 365-377 (2006)
Phospholipase D: enzymology, mechanisms of regulation, and function.
Exton JH
Physiological Reviews, 77(2), 303-320 (1997)
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