P0029
Protease from Bacillus sp.
Synonym(s):
Protamex®
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About This Item
biological source
Bacillus sp.
Quality Level
form
granular
enzyme activity
>1.5 AU-N/g
storage temp.
2-8°C
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General description
Protease from Bacillus sp. is extensively useful in pharmaceutical, leather, food and waste processing industry. It is widely used in manufacturing protein hydrolysates. Analysis of Bacillus protease stability and activity showed that the enzyme was active at all pH values from 5.0-11.0 with 100% activity at 8.0. Optimal pH for protein stability was determined to be at 7.0 while the optimal temperature for stability was at 60°C with 95% activity maintained.
Application
Protease from Bacillus sp. has been used:
- to evaluate protease inhibitor activity
- in high yields in batch fermentation using a response surface methodology
- in a study to investigate a biosensor to detect a protease biomarker from Bacillus licheniformis
Analysis Note
minimum activity 1.5 AU-N/G solid
Legal Information
Protamex is a registered trademark of Novozymes Corp.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Hypocholesterolemic effect of compounded freshwater clam protein hydrolysate and Gracilaria
Food Chemistry, 123(2), 395-399 (2010)
The Analyst, 137(20), 4829-4833 (2012-08-30)
A simple, selective and sensitive electrochemical biosensor has been developed to detect protease biomarker from Bacillus licheniformis, a recognized model of the biochemical warfare agent Bacillus anthracis. In this assay, the biosensor is constructed using a d-amine acid containing substrate
Trypsin Inhibitor from Edible Mushroom Pleurotus floridanus Active against Proteases of Microbial Origin
Applied Biochemistry and Biotechnology, 167-178 (2014)
Some properties of extracellular protease from Bacillus licheniformis LBBL-11 isolated from ?iru?, a traditionally fermented African locust bean condiment.
African Journal of Biochemistry Research, 2, 206-210 (2008)
Characterization of protein hydrolysates and lipids obtained from black scabbardfish (Aphanopus carbo) by-products and antioxidative activity of the hydrolysates produced.
Process. Biochem., 45(1), 18-24 (2010)
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