OGS387
PSF-CMV-PGK - DUAL PROMOTER EXPRESSION PLASMID
plasmid vector for molecular cloning
Synonym(s):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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About This Item
UNSPSC Code:
12352200
form
buffered aqueous solution
mol wt
size 4747 bp
bacteria selection
kanamycin
Origin of replication
pUC (500 copies)
Peptide cleavage
no cleavage
Promoter
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
reporter gene
none
shipped in
ambient
storage temp.
−20°C
General description
This cloning vector contains two promoters that terminate transcription at the same poly-adenylation signal allowing the expression of two genes from one expression cassette. The second promoter (PGK) is approximately 10-fold weaker than the upstream CMV promoter.
Promoter Expression Level: PSF-CMV-PGK - DUAL PROMOTER EXPRESSION PLASMID contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Promoter Expression Level: PSF-CMV-PGK - DUAL PROMOTER EXPRESSION PLASMID contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Application
PSF-CMV-PGK - DUAL PROMOTER EXPRESSION PLASMID contains two multiple cloning sites and allows the insertion of two genes one downstream of the CMV promoter and one downstream of the PGK promoter. Both promoters terminate transcription at the same poly-adenylation signal
Multiple Cloning Site Notes: There is a start codon in the NcoI site that can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI but the Shine-Dalgarno sequences and KOZAK ribosomal entry site sequences are aligned with the start codon in the NcoI site. The PGK promoter has been inserted between the ClaI and BamHI sites and contains sites downstream to allow a second gene to be inserted.
The BsgI and BseRI restriction sites in the first MCS cleave within the stop codon in the XbaI site and allow the retrospective fusion of coding sequences.
Multiple Cloning Site Notes: There is a start codon in the NcoI site that can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI but the Shine-Dalgarno sequences and KOZAK ribosomal entry site sequences are aligned with the start codon in the NcoI site. The PGK promoter has been inserted between the ClaI and BamHI sites and contains sites downstream to allow a second gene to be inserted.
The BsgI and BseRI restriction sites in the first MCS cleave within the stop codon in the XbaI site and allow the retrospective fusion of coding sequences.
Sequence
To view sequence information for this product, please visit the product page
Analysis Note
To view the Certificate of Analysis for this product, please visit www.oxgene.com
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Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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