OGS2891
PSF-OXB20-COOH-TEV-FLAG®-6HIS - C-TERMINAL 6 HIS AND FLAG® DUAL TAG BACTERIAL PLASMID
plasmid vector for molecular cloning
Synonym(s):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
About This Item
recombinant
expressed in E. coli
tag
6-His tagged
FLAG® tagged
form
buffered aqueous solution
mol wt
size 3917 bp
bacteria selection
kanamycin
Origin of replication
pUC (500 copies)
Peptide cleavage
TEV
Peptide tag location
C-terminal
Promoter
Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial
reporter gene
none
shipped in
ambient
storage temp.
−20°C
General description
About the Peptide Tag:This plasmid contains a c-terminal FLAG epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK. This plasmid also contains a secondary Hexa-Histidine (6His) tag protein tag. The sequence of this tag is: HHHHHHWe provide a range of dual peptide tag plasmids. This is because some peptide tags provide specific biological properties (e. g., small molecule affinity new epitopes solubility or protein secretion) that are not provided by others.
About the Cleavage Tag: PSF-OXB20-COOH-TEV-FLAG®-6HIS - C-terminal 6 HIS AND FLAG® dual tag bacterial plasmid lso encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a TEV cleavage tag. The protein sequence of the cleavage tag is: ENLYFQG. Cleavage occurs between the Glu and Gly residues. TEV is often reported to have better specificity for its recognition site compared to EKT Thrombin or Faxtor Xa.
Promoter Expression Level: This plasmid vector contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.
PSF-OXB20-COOH-TEV-FLAG® -6HIS - C-terminal 6 HIS AND FLAG® dual tag bacterial plasmid is a C-terminal 6His and FLAG dual affinity and epitope tag bacterial expression plasmid. This plasmid vector allows the creation of tagged proteins with a TEV cleavage tag.
Sequence
Analysis Note
Legal Information
related product
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
Certificates of Analysis (COA)
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