OGS1248
PSF-CMV-PURO-COOH-3C-FLAG®-6HIS - C-TERMINAL 6 HIS AND FLAG® DUAL TAG MAMMALIAN PLASMID
plasmid vector for molecular cloning
Synonym(s):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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CN¥11,407.80
Select a Size
About This Item
CN¥11,407.80
tag
6-His tagged
FLAG® tagged
form
buffered aqueous solution
mol wt
size 6216 bp
bacteria selection
kanamycin
mammalian cells selection
puromycin
Origin of replication
pUC (500 copies)
Peptide cleavage
3C
Peptide tag location
C-terminal
Promoter
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
reporter gene
none
1 of 4
This Item | 67034U | 67033U | 66825U |
|---|---|---|---|
| matrix spherical silica particle platform, superficially porous particle | matrix spherical silica particle platform, superficially porous particle | matrix spherical silica particle platform, superficially porous particle | matrix spherical silica particle platform, superficially porous particle |
| separation technique reversed phase | separation technique reversed phase | separation technique reversed phase | separation technique reversed phase |
| particle size 3.4 μm | particle size 3.4 μm | particle size 3.4 μm | particle size 3.4 μm |
| matrix active group C4 (butyl) phase | matrix active group C4 (butyl) phase | matrix active group C4 (butyl) phase | matrix active group C4 (butyl) phase |
| pore size 400 Å | pore size 400 Å | pore size 400 Å | pore size 400 Å |
| technique(s) HPLC: suitable, UHPLC-MS: suitable, LC/MS: suitable, UHPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable |
General description
About the Peptide Tag:This plasmid contains a c-terminal FLAG epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK. This plasmid also contains a secondary Hexa-Histidine (6His) tag protein tag. The sequence of this tag is: HHHHHHWe provide a range of dual peptide tag plasmids. This is because some peptide tags provide specific biological properties (e. g., small molecule affinity new epitopes solubility or protein secretion) that are not provided by others.
About the Cleavage Tag: This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a 3C cleavage tag. The protein sequence of the cleavage tag is: LEVLFQGP. Human Rhinovirus (HRV) 3C Protease is a highly specific protease that cleaves between the Glu and Gly residues of its recognition site. It is often produced with the trademname ′PreScission protease′.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Sequence
Analysis Note
Legal Information
related product
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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