OGS1080
PSF-CMV-PURO-COOH-THR-10HIS - C-TERMINAL 10HIS TAG MAMMALIAN PLASMID
plasmid vector for molecular cloning
Synonym(s):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
About This Item
tag
10-His tagged
form
buffered aqueous solution
mol wt
size 6198 bp
bacteria selection
kanamycin
mammalian cells selection
puromycin
Origin of replication
pUC (500 copies)
Peptide cleavage
thrombin
Peptide tag location
C-terminal
Promoter
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
reporter gene
none
1 of 4
This Item | OGS1119 | OGS1158 | OGS1157 |
|---|---|---|---|
| peptide tag location C-terminal | peptide tag location N-terminal | peptide tag location C-terminal | peptide tag location N-terminal |
| peptide cleavage thrombin | peptide cleavage TEV | peptide cleavage EKT | peptide cleavage EKT |
| origin of replication pUC (500 copies) | origin of replication pUC (500 copies) | origin of replication pUC (500 copies) | origin of replication pUC (500 copies) |
| mammalian cells selection puromycin | mammalian cells selection puromycin | mammalian cells selection puromycin | mammalian cells selection puromycin |
| promoter Promoter name: CMV | promoter Promoter name: CMV | promoter Promoter name: CMV | promoter Promoter name: CMV |
General description
About the Peptide Tag:This plasmid contains a c-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.
About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a Thrombin cleavage tag. The protein sequence of the cleavage tag is: LVPRGS. It cleaves preferentially between the Arg and Gly residues. Off target cleavage can often occur at non-specific sites normally from other contaminating proteases. To ensure maximal protein integrity the enzyme reagent must be highly pure.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.
Sequence
Genebank Vector Sequence File
FASTA Vector Sequence File
Full Plasmid Map
Analysis Note
Other Notes
Legal Information
related product
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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