O8389
Anti-Oct 3/4 antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
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Synonym(s):
Anti-OTF3/OTF4, Anti-Octamer-binding transcription factor 3/4, Anti-POU class 5 homeobox 1, Anti-POU5F1
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse, human, rat
concentration
~1 mg/mL
technique(s)
immunohistochemistry: 1:100-1:250
western blot: 1:500-1:1,000
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... POU5F1(5460)
mouse ... Pou5f1(18999)
rat ... Pou5f1(294562)
General description
POU domain class 5 transcription factor 1 (POU5F1) is also termed as OCT4. It is a transcription factor. OCT4 codes for three isoforms, such as OCT4A, OCT4B and OCT4B1. These isoforms are produced by alternative splicing. The OCT4 gene consists of 7 exons. This gene is located on human chromosome 6. OCT4A and OCT4B are located in the nucleus and cytoplasm respectively.
Immunogen
synthetic peptide corresponding to an internal sequence of human Oct 3/4. This sequence is 84% identical in mouse and 94% identical in rat.
Application
Anti-Oct 3/4 antibody produced in rabbit has been used in immunocytochemistry and immunofluorescence staining.
Biochem/physiol Actions
Oct 3/4 maintains developmental potency and governs distinct fates of embryonic stem cells. A moderate increase in expression causes differentiation into primitive endoderm and mesoderm. In contrast, repression of Oct 3/4 stimulates loss of pluripotency and dedifferentiation to trophectoderm. POU domain class 5 transcription factor 1 (POU5F1) serves as a master regulator of pluripotency in embryonic stem cells. It is one of the reprogramming factors, that is highly essential for generating induced pluripotent stem cells.
Physical form
solution in phosphate buffered saline, containing 0.02% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Pricing
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Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Optimization of Serum-Free Culture Conditions for Propagation of Putative Buffalo (Bubalus bubalis) Spermatogonial Stem Cells
Sharma A, et al.
Cellular Reprogramming, 21(1) (2019)
OCT4B Isoform Promotes Anchorage-Independent Growth of Glioblastoma Cells
Choi SH, et al.
Molecules and cells, 42(2), 135-135 (2019)
Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells
Niwa H, et al.
Nature Genetics, 24(4), 372-376 (2000)
Michalina Alicka et al.
Stem cell research & therapy, 11(1), 4-4 (2020-01-05)
Progressive loss of cell functionality caused by an age-related impairment in cell metabolism concerns not only mature specialized cells but also its progenitors, which significantly reduces their regenerative potential. Adipose-derived stem cells (ASCs) are most commonly used in veterinary medicine
Luca Ampollini et al.
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 46(6), e103-e112 (2014-10-15)
The aim of the present study was to characterize the biological properties and in vivo tumourigenic potential of mesenchymal cells (MCs) obtained from non-small-cell lung cancer (NSCLC) samples. NSCLC samples (53 adenocarcinomas and 24 squamous-cell carcinomas) surgically removed from 46
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