O4886
Monoclonal Anti-Opsin antibody produced in mouse
clone RET-P1, ascites fluid
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
RET-P1, monoclonal
mol wt
antigen 39 kDa by immunoblotting (IB of rat retina produces closely spaced doublet)
contains
15 mM sodium azide
species reactivity
goldfish, mouse, tiger salamander, rat, amphibian, quail, dove, duck, rabbit, bovine, human, turtle
technique(s)
electron microscopy: suitable
immunocytochemistry: suitable using cultured cells
immunohistochemistry (frozen sections): 1:10,000 using frozen sections of rat eye
indirect ELISA: suitable
radioimmunoassay: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RHO(6010)
mouse ... Rho(212541)
rat ... Rho(24717)
General description
Mouse monoclonal clone RET-P1 anti-Opsin antibody recognizes an epitope located in amino acid residues 4-10 at the N-terminus of the rhodopsin molecule. In immunoblotting of rat retina, the antibody labels a closely spaced doublet of 39 kDa and less intense bands of 78 and 115 kDa, representing rhodopsin monomer and aggregates. Higher M.W. bands are stained in pituitary (251 and 288 kDa) and in hypothalamus (269 and 331 kDa) in addition to several lower M.W. bands. The product specifically labels the cell bodies, outer and inner segments (rods but not cones) of rat photoreceptor surface. It reacts with bovine, duck, amphibian, goldfish, rabbit, rat, turtle, tiger salamander, mouse, dove, quail, and human rhodopsin/opsin.
Opsin belongs to rhodopsin receptor. The gene is located on human chromosome 3q22.1. Opsin gene is mainly expressed in rod cells.
Photoreceptors are responsible for the initial step in visual processing, converting the signal of photon absorption into synaptic transmission. The visual pigment in vertebrate rods responsible for the absorption of light quanta is rhodopsin (also called opsin). Rhodopsin comprises >95% of the rod outer segments (ROS) intrinsic membrane protein and is a glycoprotein possessing two asparagine-linked oligosaccharide groups at amino acid residues 2 and 15 of the N-terminus. Light is absorbed by rhodopsin and the subsequent conformational change leads to the activation of a cyclic GMP phosphodiesterase through a specific G-protein intermediate, transducin.
Immunogen
rat retinal membranes.
Application
Monoclonal Anti-Opsin antibody produced in mouse has been used in immunofluorescence and histology.
Mouse monoclonal clone RET-P1 anti-Opsin antibody is used to tag opsin/rhodopsin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting, immunocytochemistry of cultured cells, immunohistochemistry (paraformaldehyde/glutaraldehyde-fixed, paraformaldehyde perfusion-fixed, frozen sections), immunoelectron microscopy, ELISA, competitive ELISA, and solid phase RIA. It is used as a probe to determine the presence and roles of opsin/rhodopsin in studies of rhodopsin location, functional properties and molecular mechanisms governing rod photoreceptor differentiation.
Biochem/physiol Actions
Mutations in rhodopsin gene are linked to retinitis pigmentosa (RP) and congenital night blindness.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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Autologous transplantation of RPE with partial-thickness choroid after mechanical debridement of Bruch membrane in the rabbit
Hu Y, et al.
Investigative Ophthalmology & Visual Science, 49(7), 3185-3192 (2008)
Monoclonal antibodies which recognize different cell types in the rat retina
Barnstable, C.J.
Nature, 286, 231-231 (1980)
Rhodopsin mutation G90D and a molecular mechanism for congenital night blindness
Rao VR, et al.
Nature, 367(6464), 639-639 (1994)
Nuclear architecture of rod photoreceptor cells adapts to vision in mammalian evolution
Solovei I, et al.
Cell, 137(2), 356-368 (2009)
Sutisak Kitareewan et al.
International journal of oncology, 33(2), 397-404 (2008-07-19)
All-trans-retinoic acid (RA) treatment of acute promyelocytic leukemia (APL) cases expressing the t(15;17) product, PML/RARalpha, is a successful example of differentiation therapy. Uncovering RA target genes is of considerable interest in APL. This study comprehensively examines in APL cells transcriptional
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