O3131
Oligo(dT)-Cellulose
For polyadenylated mRNA purification
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About This Item
MDL number:
UNSPSC Code:
12352200
foreign activity
RNase, none detected
storage temp.
−20°C
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Application
For polyadenylated mRNA purification using rapid and simple chromatographic techniques. Covalently attached oligothymidylic acid chains are approx. 20 nucleotides long. Eukaryotic mRNA specifically binds by its 3′-poly(A) tail. Non-polyadenylated RNA and other contaminants can be removed by washing, and the purified mRNA is eluted with a low salt buffer.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Nikolay E Shirokikh et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(31), 10738-10743 (2008-07-29)
Eukaryotic mRNAs in which a poly(A) sequence precedes the initiation codon are known to exhibit a significantly enhanced cap-independent translation, both in vivo and in cell-free translation systems. Consistent with high expression levels of poxviral mRNAs, they contain poly(A) sequences
A Krystosek et al.
The Journal of biological chemistry, 250(15), 6077-6084 (1975-08-10)
The polyadenylic acid-containing messenger ribonucleic acids of eukaryotic cells are rapidly isolated and deproteinized in a simple and gentle one-step procedure. The polyribosome fraction, dissolved in 0.5 M NaCl/0.5 percent sodium dodecyl sulfate, is passed through an oligo(dT)-cellulose column which
H Aviv et al.
Proceedings of the National Academy of Sciences of the United States of America, 69(6), 1408-1412 (1972-06-01)
A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologically active
Gilham
Journal of the American Chemical Society, 86, 4982-4982 (1964)
Peter G Fuerst et al.
Neuron, 64(4), 484-497 (2009-12-01)
DSCAM and DSCAM-LIKE1 (DSCAML1) serve diverse neurodevelopmental functions, including axon guidance, synaptic adhesion, and self-avoidance, depending on the species, cell type, and gene family member studied. We examined the function of DSCAM and DSCAML1 in the developing mouse retina. In
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