NA0400
GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit
sufficient for 10 preparations
Sign Into View Organizational & Contract Pricing
All Photos(2)
Synonym(s):
Gen Elute, GenElute Endotoxin-Free Plasmid Kit
UNSPSC Code:
41105501
NACRES:
NA.52
Recommended Products
usage
sufficient for 10 preparations
Quality Level
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA extraction: suitable
greener alternative category
storage temp.
15-25°C
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit offers a simple and rapid method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. The kit uses a vacuum format with a filter column for the rapid clearing of the bacterial lysate and a silica column for capturing plasmid DNA. A proprietary solution binds plasmid DNA to the binding column while preventing endotoxins from adsorbing. The technology allows for the user to consistently achieve levels of endotoxin that are less than 0.1 endotoxin units per μg of plasmid DNA.
High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells.
High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Features and Benefits
- Up to 1.2 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
- Only 35 minutes from pelleted cells to purified plasmid
- Convenient vacuum format
Principle
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is clarified by filtration followed by the addition of a binding solution that has been optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured on silica, while endotoxins are prevented from adsorbing to the membrane. Two wash steps remove contaminants. Finally, the bound DNA is eluted in endotoxin-free water. The recovered plasmid DNA is predominately in its supercoiled form. Genomic DNA and RNA are below detectable levels by ethidium bromide stained agarose gel electrophoresis.
Other Notes
For additional information, please see www.sigma-aldrich.com/genelutehp.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
related product
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Central nervous system
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
动植物源性产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Dale Cowley et al.
eLife, 4 (2015-09-04)
The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010
Keith Hansen et al.
Journal of visualized experiments : JoVE, (64)(64), e3304-e3304 (2012-06-27)
Genome editing is a powerful technique that can be used to elucidate gene function and the genetic basis of disease. Traditional gene editing methods such as chemical-based mutagenesis or random integration of DNA sequences confer indiscriminate genetic changes in an
Paul A Beare et al.
Journal of bacteriology, 191(5), 1369-1381 (2008-12-31)
Coxiella burnetii is a gram-negative obligate intracellular bacterium and the causative agent of human Q fever. The lack of methods to genetically manipulate C. burnetii significantly impedes the study of this organism. We describe here the cloning and characterization of
João M Furtado et al.
Investigative ophthalmology & visual science, 53(11), 6856-6862 (2012-09-07)
Toxoplasma gondii, the parasite responsible for ocular toxoplasmosis, accesses the retina from the bloodstream. We investigated the dendritic cell as a potential taxi for T. gondii tachyzoites moving across the human retinal endothelium, and examined the participation of adhesion molecules
Ming-Wei Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(36), 13538-13543 (2008-09-04)
H5N1 influenza viruses have spread extensively among wild birds and domestic poultry. Cross-species transmission of these viruses to humans has been documented in over 380 cases, with a mortality rate of approximately 60%. There is great concern that a H5N1
Related Content
GenElute HP Plasmid Maxiprep Kit isolates endotoxin-free plasmid DNA in 35 minutes, meeting industry standards.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service