N9284
Nitroreductase from Escherichia coli
≥90% (SDS-PAGE), recombinant, expressed in E. coli
Synonym(s):
NTRA
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About This Item
Specific activity:
≥100 units/mL
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
assay
≥90% (SDS-PAGE)
form
lyophilized powder
specific activity
≥100 units/mL
mol wt
monomer 24000
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UniProt accession no.
greener alternative category
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
Escherichia coli K12 ... nfsB(945483)
Related Categories
General description
Lyophilized powder containing PBS. Does not contain BSA as excipient
Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer, with 217 amino acids and active site in each subunit. The structure has FMN and the substrate bound to the enzyme.
Application
Nitroreductase from Escherichia coli has been used in the conjugation generation with pig liver esterase (PLE). It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes.
Biochem/physiol Actions
Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones. The mutant F124K of NTR is useful in cancer therapy and improves sensitization of drug CB1954.
Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.
Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.
Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.
Preparation Note
Produced using animal component-free materials.
Other Notes
One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Sébastien Jenni et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 248, 119179-119179 (2020-11-30)
The ever-growing demand for fluorogenic dyes usable in the rapid construction of analyte-responsive fluorescent probes, has recently contributed to a revival of interest in the chemistry of diketopyrrolopyrrole (DPP) pigments. In this context, we have explored the potential of symmetrical
G M Anlezark et al.
Biochemical pharmacology, 50(5), 609-618 (1995-08-25)
A nitroreductase isolated and purified from Escherichia coli B has been demonstrated to have potential applications in ADEPT (antibody-directed enzyme prodrug therapy) by its ability in vitro to reduce dinitrobenzamides (e.g. 5-aziridinyl 2,4-dinitrobenzamide, CB 1954 and its bischloroethylamino analogue, SN
Structural and Mechanistic Studies of Escherichia coli Nitroreductase with the Antibiotic Nitrofurazone REVERSED BINDING ORIENTATIONS IN DIFFERENT REDOX STATES OF THE ENZYME
Race PR, et al.
The Journal of Biological Chemistry, 280(14), 13256-13264 (2005)
Kinetics-Based Measurement of Hypoxia in Living Cells and Animals Using an Acetoxymethyl Ester Chemiluminescent Probe
Ryan LS, et al.
ACS sensors (2019)
Nitroreductase (GlNR1) increases susceptibility of Giardia lamblia and Escherichia coli to nitro drugs.
Nillius, D. J.
Antimicrobial Agents and Chemotherapy, 66, 1029-1035 (2011)
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