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N8630

Sigma-Aldrich

Nuclease P1 from Penicillium citrinum

lyophilized powder, ≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)

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Synonym(s):
3′-Phosphohydrolase, Nuclease 5′-Nucleotidehydrolase, Endonuclease P1
CAS Number:
54576-84-0
Enzyme Commission number:
3.1.30.1 (BRENDA, IUBMB)
MDL number:
MFCD00131010
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Penicillium citrinum

Quality Level

200

form

lyophilized powder

specific activity

≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)

secondary activity

≥1,000 units/mg protein 3′-nucleotidase

mol wt

42-50 kDa

packaging

vial of ≥250 units (using RNA substrate)

technique(s)

DNA extraction: suitable
DNA purification: suitable

suitability

suitable for molecular biology

application(s)

cell analysis

storage temp.

2-8°C

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General description

Nuclease P1 is one of the most commonly known single-strand specific nucleases in molecular biology; Nuclease P1 is a single stranded specific endoduclease (of ssDNA or ssRNA). Nuclease P1 can also cleave single-stranded regions in double-stranded nucleic acids.
Nuclease P1 from Penicillium citrinum is a zinc-dependent endonuclease that exhibits increased activity in the presence of low concentrations of urea. Nuclease P1 selective activity has found useful applications in studies on nucleic acid structure.

Application

  • Nuclease P1 cleave of single stranded DNA or RNA to 5′ mononucleotides
  • Nuclease P1 supports DNA damage and modification research
  • Nucleic acids base composition and structural analysis can be done by Nuclease P1
  • Nuclease P1 has historically been used for the industrial production of 5′-mononucleotides from yeast RNA.
  • Removal of nucleic acids through protein purification can be done by Nuclease P1
  • Nuclease P1 is a key reagent for the development of methods for studies involving t-RNA dependent amino acid biosynthesis and t-RNA dependent trans-amidation
  • Nuclease P1 from Penicillium citrinum has been used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent.
  • Nuclease P1 was used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5′ or 3′ end group labeled RNA.
  • Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts.
The enzyme has an optimal temperature of approximately 70°C, but for a long incubation, a temperature below 60°C is more suitable. It is stable in the pH range of 5 - 8.
The enzyme has an optimal temperature of approximately 70 °C, but for a long incubation, a temperature below 60 °C is more suitable. It is stable in the pH range of 5 - 8.

Biochem/physiol Actions

Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phosphooligonucleotides. It does not appreciably degrade double-stranded nucleic acids, especially in the presence of more than 400 mM sodium chloride at pH 6.0.

Features and Benefits

Our highly active Nuclease P1 is tested for its 3′- 5′ - Phosphodiesterase Activity and 3′- Nucleotidase Activity and is the most active Nuclease P1 in the market

Physical properties

A zinc dependent glycoprotein consisting of 270 amino acid residues. Molecular mass: 42-50 kDa.

Unit Definition

3′-5′-Phosphodiesterase: One unit will liberate 1.0 μmole of acid soluble nucleotides from RNA per min at pH 5.3 at 37 °C.
3′-Nucleotidase: One unit will hydrolyze 1.0 μmole of orthophosphate from 3′-AMP per min at pH 7.2 at 37 °C.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Product N8630 (Nuclease P1 from Penicillium citrinum is a lyophilized powder. How should I store solutions of this enzyme?

    A solution of Nuclease P1 that has been reconstituted in 20 mM sodium acetate buffer (pH 5.3), 5 mM ZnCl2, and 50 mM sodium chloride can be stored for the short term at 2-8 °C for 2-3 weeks. For longer term storage, at -20 °C, one suggestion is to add 40-50% glycerol after reconstitution in the aqueous buffer. Such solutions (with glycerol added) may be stored for 3-6 months at -20 °C.

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Fahimeh Salehi et al.
Scientific reports, 8(1), 13902-13902 (2018-09-19)
DNA targeting anticancer agents have been very successful in clinic, especially, when used in combinatorial therapy. But unfortunately, they often exhibit high levels of toxicity towards normal cells. Hence, much effort has been put into finding agents with more selectivity
Fiona J Flett et al.
Nature communications, 9(1), 24-24 (2018-01-04)
Tyrosyl-DNA phosphodiesterase (Tdp1) is a DNA 3'-end processing enzyme that repairs topoisomerase 1B-induced DNA damage. We use a new tool combining site-specific DNA-protein cross-linking with mass spectrometry to identify Tdp1 interactions with DNA. A conserved phenylalanine (F259) of Tdp1, required
Xiaohuan Jin et al.
Nucleic acids research, 47(2), 883-898 (2018-12-07)
Modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m1G) on position 37; a function mediated by Trm5 homologs. We show that AtTRM5a (At3g56120) is a Trm5 ortholog in Arabidopsis thaliana.
A Lahm et al.
Journal of molecular biology, 215(2), 207-210 (1990-09-20)
P1 nuclease, a zinc-dependent single-strand specific endonuclease from Penicillium citrinum, has been crystallized in three different space groups using either ammonium sulphate or polyethylene glycol 4000 as the precipitating agent. The crystals diffract to between 3 A and 2.2 A.
M V Reddy et al.
Carcinogenesis, 7(9), 1543-1551 (1986-09-01)
Exceedingly sensitive procedures are required to detect the presence of covalent DNA adducts in humans exposed to environmental genotoxicants because of low levels of such derivatives (1 adduct in 10(8)-10(10) DNA nucleotides). A 32P-postlabeling assay for detection and quantitation of
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