N8523
Monoclonal Anti-NF-κB antibody produced in mouse
clone NF-12, ascites fluid
Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
Anti-CVID12, Anti-EBP-1, Anti-KBF1, Anti-NF-kB, Anti-NF-kB1, Anti-NF-kappa-B1, Anti-NF-kappaB, Anti-NF-kappabeta, Anti-NFKB-p105, Anti-NFKB-p50, Anti-NFkappaB
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
NF-12, monoclonal
mol wt
antigen 65 kDa
contains
15 mM sodium azide
species reactivity
human, mouse
technique(s)
immunocytochemistry: suitable
microarray: suitable
western blot: 1:1,000 using a whole cell extract from a mouse fibroblast cell line
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NFKB1(4790)
mouse ... Nfkb1(18033)
General description
Monoclonal Anti-NF-κB p65 (mouse IgG1 isotype) is derived from the NF-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant, mouse NF-κB p65 fragment. Nuclear factor κB (NF-κB1) is a member of NF-κB/Rel family of transcription factors.
Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (NFKB1) is a subunit of nuclear factor κB (NF-κB) and exists as a dimer. It is expressed in the cytosol.
Immunogen
recombinant mouse NF-κB p65 fragment (C-terminal, 151 amino acids).
Application
Monoclonal Anti-NF-κB antibody produced in mouse has been used in immunohistochemistry and Western blotting.
Biochem/physiol Actions
Nuclear factor kappa-B (NF-κB1) has an ability to affect transcription of κB reporter genes positively or negatively when assayed following transfection.
Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (NFKB1) has a role in hippocampal neurogenesis. It regulates the signaling by nuclear factor κB (NF-κB). Deletion of the gene in mice leads to defects in innate and adaptive immunity. The protein acts as a mediator of apoptotic response to S-phase DNA damage and replication stress. NFKB1 modulates the structural plasticity of cortical neurons. Knockout of the protein in mice has been shown to cause liver apoptosis and embryonic death.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
recommended
Product No.
Description
Pricing
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
NF-kappaB family of transcription factors: central regulators of innate and adaptive immune functions
Caamano J and Hunter C A
Clinical Microbiology Reviews, 15(3), 414-429 (2002)
Cheng Lin et al.
Experimental and therapeutic medicine, 11(4), 1355-1360 (2016-04-14)
The aim of the current study was to observe the effects of simvastatin and taurine on delayed cerebral vasospasm (DCVS) following experimental subarachnoid hemorrhage (SAH) in rabbits. A total of 48 New Zealand white rabbits were allocated at random into
Min Hu et al.
International journal of clinical and experimental pathology, 11(4), 2072-2079 (2018-04-01)
In the establishment and continuation of a successful pregnancy, the proliferation, migration, and invasion of trophoblast cells play essential roles. Impaired trophoblast function has been reported to be involved in recurrent spontaneous abortion (RSA) but the potential mechanisms are still
Effects of simvastatin and taurine on delayed cerebral vasospasm following subarachnoid hemorrhage in rabbits
Cheng Lin
Experimental and Therapeutic Medicine, 1355-1360 (2016)
Cortical Structure Alterations and Social Behavior Impairment in p50-Deficient Mice
Sara Anna Bonini
Cerebral Cortex, 26(6), 2832?2849-2832?2849 (2016)
Articles
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service