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N8267

Sigma-Aldrich

Nitrocellulose membranes for blotting

pore size 0.45 μm, size 15 cm × 15 cm

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About This Item

MDL number:
MFCD00081525
UNSPSC Code:
12352200

size

15 cm × 15 cm

pore size

0.45 μm

SMILES string

OC[C@@H]1O[C@@H](O[C@H]2[C@@H](O)[C@H](O)[C@@H](O)O[C@H]2CO)[C@@H](O)[C@H](O)[C@H]1O.[O-][N+](=O)OC[C@@H]3O[C@H](O[N+]([O-])=O)[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H]3O[C@H]4O[C@@H](CO[N+]([O-])=O)[C@@H](O[N+]([O-])=O)[C@@H](O[N+]([O-])=O)[C@@H]4O[N+]([O-])=O

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Application

Nitrocellulose membranes are the most popular membranes for immunoblotting (Western blotting) of proteins. The basis of protein binding to nitrocellulose is believed to be due to hydrophobic interactions. The 0.45 μm pore size is the most commonly used for proteins greater than 10,000 MW, but for smaller proteins 0.2 μm is recommended to more efficiently trap the proteins.

recommended

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Danger

Hazard Statements

H228

Precautionary Statements

P210

Hazard Classifications

Flam. Sol. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

新产品

Certificates of Analysis (COA)

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Agnieszka Mykowska et al.
Nucleic acids research, 39(20), 8938-8951 (2011-07-29)
Mutant transcripts containing expanded CUG repeats in the untranslated region are a pathogenic factor in myotonic dystrophy type 1 (DM1). The mutant RNA sequesters the muscleblind-like 1 (MBNL1) splicing factor and causes misregulation of the alternative splicing of multiple genes
Alison G Greene et al.
Experimental eye research, 201, 108349-108349 (2020-11-15)
Pseudoexfoliation syndrome (PXF) is the most common cause of secondary open angle glaucoma worldwide. Single nucleotide polymorphisms (SNPs) in the gene Lysyl oxidase like 1 (LOXL1) are strongly associated with the development of pseudoexfoliation glaucoma (PXFG). However, these SNPs are
Sunniva Stordal Bjørklund et al.
BMC cancer, 15, 524-524 (2015-07-18)
Alternate transcripts from a single gene locus greatly enhance the combinatorial flexibility of the human transcriptome. Different patterns of exon usage have been observed when comparing normal tissue to cancers, suggesting that variant transcripts may play a role in the

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