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N7255

Sigma-Aldrich

1-Naphthyl phosphate disodium salt

phosphatase substrate, fluorogenic, powder

Synonym(s):

α-Naphthyl phosphate disodium salt, Disodium 1-naphthyl phosphate

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About This Item

Empirical Formula (Hill Notation):
C10H7Na2O4P
CAS Number:
2183-17-7
Molecular Weight:
268.11
EC Number:
218-564-3
MDL number:
MFCD00041007
UNSPSC Code:
12352204
PubChem Substance ID:
24897818
NACRES:
NA.32

product name

1-Naphthyl phosphate disodium salt, phosphatase substrate

Quality Level

200

form

powder

composition

phosphorous, 11.2-12.4% ICP (anhydrous)
sodium, 16.0-18.4% ICP (anhydrous)

solubility

water: 50 mg/mL, clear, colorless to very faintly yellow

storage temp.

−20°C

SMILES string

[Na+].[Na+].[O-]P([O-])(=O)Oc1cccc2ccccc12

InChI

1S/C10H9O4P.2Na/c11-15(12,13)14-10-7-3-5-8-4-1-2-6-9(8)10;;/h1-7H,(H2,11,12,13);;/q;2*+1/p-2

InChI key

QYURIFWAOPAPAJ-UHFFFAOYSA-L

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Application

1-Naphthyl phosphate disodium salt has been used:
  • as a component in the mixture for soaking the chromatography paper for determining acid phosphatase activity
  • in the staining of the alkaline phosphatase isoforms in 7.5% non-denaturing polyacrylamide gel
  • as a component in the mixture for soaking the chromatography paper for preparing acid phosphomonoesterase-reactive imprinting sheets
  • as a component in the staining solution for staining the roots for determining phosphomonoesterase activity

Substrates

Substrate for determination of phosphatase.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Improving phosphorus acquisition of white clover (Trifolium repens L.) by transgenic expression of plant-derived phytase and acid phosphatase genes
Ma XF, et al.
Plant Science, 176(4), 479-488 (2009)
The net charge of the first 18 residues of the mature sequence affects protein translocation across the cytoplasmic membrane of gram-negative bacteria
Kajava AV, et al.
Journal of Bacteriology, 182(8), 2163-2169 (2000)
Nambirajan Rangarajan et al.
The Journal of biological chemistry, 295(41), 14189-14202 (2020-08-14)
Autophagy is a conserved process that recycles cellular contents to promote survival. Although nitrogen limitation is the canonical inducer of autophagy, recent studies have revealed several other nutrients important to this process. In this study, we used a quantitative, high-throughput
Control of soil phosphatase activities at millimeter scales in a mixed paper birch-Douglas-fir forest: The importance of carbon and nitrogen
Godin AM, et al.
Soil Biology and Biochemistry, 80, 62-69 (2015)
A method for linking in situ activities of hydrolytic enzymes to associated organisms in forest soils
Dong S, et al.
Soil Biology and Biochemistry, 39(9), 2414-2419 (2007)
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