All Photos(1)
Synonym(s):
β-Naphthyl acetate
Linear Formula:
CH3CO2C10H7
CAS Number:
Molecular Weight:
186.21
Beilstein:
1868291
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32
Recommended Products
form
powder
Quality Level
impurities
<0.2% Free naphthol
mp
67-70 °C (lit.)
solubility
ethanol: 50 mg/mL
storage temp.
−20°C
SMILES string
CC(=O)Oc1ccc2ccccc2c1
InChI
1S/C12H10O2/c1-9(13)14-12-7-6-10-4-2-3-5-11(10)8-12/h2-8H,1H3
InChI key
RJNPPEUAJCEUPV-UHFFFAOYSA-N
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Application
2-Naphthyl acetate has been used as a substrate:
- in substrate sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to examine the composition of lipases in larvae and adult specimens
- in the preparation of zymograms to identify proteins with lipase activity
- to measure non-specific esterase activity and β-esterase enzyme activity
Biochem/physiol Actions
2-Naphthyl acetate/β-naphthyl acetate (BNA) is an ester substrate. It can be used in staining gels to detect esterase activity. BNA dissolved in acetone and ethanol produces a dark and very light/no background respectively. Hence destaining is not necessary if ethanol is used to dissolve BNA for esterase staining of gels.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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