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N5632

Nile Blue A

Name: Nile Blue A
Brand: SIGMA

Dye content ≥75 %, Powder

Synonym(s):

Basic Blue 12, Nile blue sulfate

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About This Item

Linear Formula:

2C₂₀H₂₀N₃O · SO₄

CAS Number:

3625-57-8

Molecular Weight:

732.85

NACRES:

NA.47

PubChem Substance ID:

24897743

UNSPSC Code:

12171500

EC Number:

222-832-5

MDL number:

MFCD00064529

Colour Index Number:

51180

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Properties

Product Name

Nile Blue A, Dye content ≥75 %

form

powder

Quality Level

100

composition

Dye content, ≥75%

mp

>300 °C (dec.) (lit.)

solubility

H₂O: 1 mg/mL, blue

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[O-]S([O-])(=O)=O.CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34.CC\[N+](CC)=C5/C=CC6=Nc7c(OC6=C5)cc(N)c8ccccc78

InChI

1S/2C20H19N3O.H2O4S/c2*1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h2*5-12,21H,3-4H2,1-2H3;(H2,1,2,3,4)

InChI key

QIRDPEPUXNCOLD-UHFFFAOYSA-N

Description

General description

Nile Blue A is aminodiethylaminonaphthophenoxazonium sulfate employed in biology and histology. It is a highly fluorescent, and photostable organic dye used to dye clothing, and is for use in lipid histochemistry.

Application

Nile blue A is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections.

Biochem/physiol Actions

Nile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red.

Features and Benefits

Used in the preparation of an amperometric glucose sensor.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Global Trade Item Number

SKU	GTIN
N5632-25G	04061834117117