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Merck
CN

N5632

Nile Blue A

Dye content ≥75 %, Powder

Synonym(s):

Basic Blue 12, Nile blue sulfate

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About This Item

Linear Formula:
2C20H20N3O · SO4
CAS Number:
3625-57-8
Molecular Weight:
732.85
NACRES:
NA.47
PubChem Substance ID:
24897743
UNSPSC Code:
12171500
EC Number:
222-832-5
MDL number:
MFCD00064529
Colour Index Number:
51180

Key Documents

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Product Name

Nile Blue A, Dye content ≥75 %

InChI key

QIRDPEPUXNCOLD-UHFFFAOYSA-N

InChI

1S/2C20H19N3O.H2O4S/c2*1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h2*5-12,21H,3-4H2,1-2H3;(H2,1,2,3,4)

SMILES string

[O-]S([O-])(=O)=O.CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34.CC\[N+](CC)=C5/C=CC6=Nc7c(OC6=C5)cc(N)c8ccccc78

form

powder

composition

Dye content, ≥75%

mp

>300 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL, blue

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

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Related Categories

Application

Nile blue A is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections.

Biochem/physiol Actions

Nile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red.

Features and Benefits

Used in the preparation of an amperometric glucose sensor.

General description

Nile Blue A is aminodiethylaminonaphthophenoxazonium sulfate employed in biology and histology. It is a highly fluorescent, and photostable organic dye used to dye clothing, and is for use in lipid histochemistry.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS7519
SHBR4222
SHBR8104
SHBS2745
SHBR0466

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Fgfr1 regulates patterning of the pharyngeal region.
Trokovic N
Genes & Development, 17, 141-141 (2003)
Segmental origin and migration of neural crest cells in the hindbrain region of the chick embryo.
Lumsden A
Development, 113, 1281-1281 (1991)
Biochemical, histochemical and ultrastructural characterization of Centrolobium robustum (Fabaceae) seeds.
Pinzon-Torres JA
Hoehnea, 36, 149-149 (2009)
Rost FWD
Fluorescence Microscopy (1992)
Jason D Slinker et al.
Nature chemistry, 3(3), 228-233 (2011-02-22)
Molecular wires show promise in nanoscale electronics, but the synthesis of uniform, long conductive molecules is a significant challenge. Deoxyribonucleic acid (DNA) of precise length, by contrast, is synthesized easily, but its conductivity over the distances required for nanoscale devices
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