N5408
Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in mouse
clone ME20.4, ascites fluid
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Synonym(s):
Anti-NGFR p75
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
ME20.4, monoclonal
mol wt
antigen 75 kDa
contains
15 mM sodium azide
species reactivity
racoon, monkey, pig, human, feline
should not react with
mouse, chicken, rat
technique(s)
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human tongue
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
neutralization: suitable
radioimmunoassay: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NGFR(4804)
Related Categories
General description
Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) (mouse IgG1 isotype) is derived from the ME20.4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a human melanoma cell line WM245. The human NGFR p75 has a hydrophobic signal sequence, a single N-linked glycosylation site, four cysteine-rich repeat units of approximately 40 amino acids in the extracellular domain, a serine- and threonine-rich region which might be O-linked glycosylated, a single transmembrane domain, and a 155-amino acid cytoplasmic domain.
Recognizes the low affinity human NGFR p75 (also known as gp75NGFR , p75NGFR and p75NTR). The epitope recognized by the antibody exists within the third or fourth cysteine-rich repeats of the NGFR molecule.
Specificity
Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) recognizes the low affinity NGFR p75 of human origin (75 kDa), also known as gp75NGFR, p75NGFR and p75NTR .The epitope recognized by the antibody is located within the third or fourth cysteine-rich repeats of the NGFR molecule. Cross-reactivity has been observed with human, monkey (marmoset), pig, cat and raccoon , but not with rat, chicken or mouse NGFR.
Immunogen
human melanoma cell line WM245.
Application
Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in mouse has been used in immunoblotting. to added in CB.
Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) may be used:
- for enzyme linked immune sorbent assay (ELISA)
- immunoprecipitation, immunoblotting and immunohistochemistry
- for immunocytochemistry (acetone- or formalin-fixed, Bouin′s solution - or formalin- fixed paraffin-embedded)
- for electron- microscopy radioimmunoassay (RIA) (Kd of 7x10-10 M)
- flow cytometry
- for the inhibition of binding of NGF to the receptor
- for rossette assay
- as a cholinergic immunotoxin
Biochem/physiol Actions
Nerve Growth Factor Receptor (NGFR) p75 enhances the NGF binding affinity of the proto-oncogene product p140trk and also may modulate the kinase activity of p140trk and play a role in signal transduction. Specifically, neurotrophin binding to NGFR p75 stimulates generation of ceramide, activation and translocation of nuclear factor- κ (NF-κB) to the nucleus, and enhancement of Jun kinase (JNK) activity.
Neurotrophins (NT) are growth factors that are essential for the development and maintenance of the nervous system. One of the receptors that mediate the effects of NTs is the Nerve Growth Factor Receptor/TNFRSF16 (p75NGFR, p75NTR). NGFR mediates the effects of NGF that are important in the survival and differentiation of neurons and neural crest-derived cells, during embryonic and early postnatal development. Expression of NGFR has been associated with favorable prognosis and survival in breast and pancreatic cancers.
Physical form
The product is supplied as ascites fluid with 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
related product
Product No.
Description
Pricing
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Barbara Kofler et al.
International journal of molecular sciences, 19(4) (2018-04-13)
Photodynamic therapy (PDT) is suggested to have an impact on the treatment of early stage head and neck cancers (HNSCC). We investigated the effect of PDT with methylene blue (MB) and a diode laser (660 nm) as the laser source
Expression and structure of the human NGF receptor
Johnson D, et al.
Cell, 47(4), 545-554 (1986)
High affinity nerve growth factor binding displays a faster rate of association than p140trk binding. Implications for multi-subunit polypeptide receptors.
Mahadeo D, et al.
The Journal of Biological Chemistry, 269(9), 6884-6891 (1994)
Common olfactory ensheathing glial markers in the developing human olfactory system
Oprych K, et al.
Brain Structure &Amp; Function, 222, 1877-1895 (2017)
Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies.
A H Ross et al.
Proceedings of the National Academy of Sciences of the United States of America, 81(21), 6681-6685 (1984-11-01)
The nerve growth factor (NGF) receptor was characterized by using a new series of anti-receptor monoclonal antibodies (MAbs). These MAbs (i) showed significantly greater reactivity with a melanoma cell line expressing higher levels of NGF receptor, (ii) inhibited the binding
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