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N4645

4-Nitrophenyl phosphate disodium salt hexahydrate

Name: 4-Nitrophenyl phosphate disodium salt hexahydrate
Brand: SIGMA

powder, BioReagent, suitable for cell culture, ≥97%

Synonym(s):

p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate

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About This Item

Linear Formula:

O₂NC₆H₄OP(O)(ONa)₂· 6H₂O

CAS Number:

333338-18-4

Molecular Weight:

371.14

Beilstein:

5324661

EC Number:

224-246-5

MDL number:

MFCD00066288

UNSPSC Code:

12352204

PubChem Substance ID:

24897708

NACRES:

NA.75

biological source

synthetic (organic)

Quality Level

200

product line

BioReagent

Assay

≥97%

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H₂O: 100 mg/mL

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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Related Categories

Phosphates

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:

as a component in diethanolamine buffer to detect alkaline phosphatase-conjugated antibodies in enzyme linked immunosorbent assay (ELISA)
to treat adherent cells in adhesion assay
as a phosphatase substrate in in vitro phosphatase assay in substrate buffer for ELISA

Biochem/physiol Actions

p-Nitrophenyl phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in enzyme linked immunosorbent assay (ELISA) procedures. It is the substrate of choice in alkaline phosphatase systems due to its high sensitivity. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. This product is tested for use in cell culture applications. 4-nitrophenyl phosphate (pNPP), a small-molecule substrate can be used to study on alkaline phosphatases, acid phosphatases etc.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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