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N3755

Sigma-Aldrich

Nuclease micrococcal from Staphylococcus aureus

100-300 units/mg protein

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Synonym(s):
Micrococcal nuclease, Endonuclease micrococcal, MNase, Micrococcal endonuclease
CAS Number:
9013-53-0
Enzyme Commission number:
3.1.31.1 (BRENDA, IUBMB)
EC Number:
232-748-0
MDL number:
MFCD00131731
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Staphylococcus aureus

Quality Level

300

form

lyophilized powder

specific activity

100-300 units/mg protein

composition

Protein, ≥40% E1%/280 (Balance primarily sodium citrate)

technique(s)

DNA purification: suitable

suitability

suitable for molecular biology

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

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Application

Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases.

Biochem/physiol Actions

Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C.
Hydrolyzes 5′-phosphodiester bonds of DNA.

Unit Definition

One unit will produce 1.0 μmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on EM/260 = 10,000 for the mixed nucleotides.

Other Notes

Note: One μmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a ΔA260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

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C Dingwall et al.
Nucleic acids research, 9(12), 2659-2673 (1981-06-25)
The substrate specificity of micrococcal nuclease (EC 3.1.4.7.) has been studied. The enzyme recognises features of nucleotide composition, nucleotide sequence and tertiary structure of DNA. Kinetic analysis indicates that the rate of cleavage is 30 times greater at the 5'
Yu Hu et al.
Foodborne pathogens and disease, 9(3), 265-271 (2012-03-03)
Thermonuclease is known as a specific virulence factor in Staphylococcus aureus. It is widely used as a genetic marker for detection of S. aureus in various types of food. Previous studies have revealed the existence of two functional thermostable nucleases
Coagulase and Heat-resistant Nuclease Producing Staphylococcus epidermidis Strains from Animals
DEVRIESE, L. and P. OEDING
Journal of Applied Microbiology, 39, 197-207 (1975)
Oliver Rothfuss et al.
Human molecular genetics, 18(20), 3832-3850 (2009-07-21)
Mutations in the parkin gene are the most common cause of recessive familial Parkinson disease (PD). Parkin has been initially characterized as an ubiquitin E3 ligase, but the pathological relevance of this activity remains uncertain. Recently, an impressive amount of
Frank J Hernandez et al.
Nature medicine, 20(3), 301-306 (2014-02-04)
Technologies that enable the rapid detection and localization of bacterial infections in living animals could address an unmet need for infectious disease diagnostics. We describe a molecular imaging approach for the specific, noninvasive detection of S. aureus based on the
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