N3518
NZ Amine® Broth
Lambda bacteriophage growth medium
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About This Item
UNSPSC Code:
12352116
NACRES:
NA.85
grade
Molecular Biology
Quality Level
sterility
non-sterile
form
powder
composition
MgSO4, 1 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
pH
6.3-7.2(1.6% solution)
application(s)
food and beverages
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
Related Categories
General description
NZ Amine is a growth medium used for the cultivation of lambda bacteriophage. This nutrient-rich broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Application
Suitable for non-selective cultivation of lambda bacteriophage.
Features and Benefits
NZ Amine powder provides:
- Standard formulation
- Convenient package sizes
- Easy scale-up using larger package sizes
- A budget-friendly alternative to liquid
Preparation Note
1. Suspend 15.94 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.
2. Autoclave for 15 minutes at 121°C.
Stir to suspend 16 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Legal Information
N-Z-Amine is a registered trademark of Kerry Group
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
监管及禁止进口产品
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Difco Manual, 356-356 (1998)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, A-A (1989)
F R Blattner et al.
Science (New York, N.Y.), 196(4286), 161-169 (1977-04-08)
The Charon lambda bacteriophages have been developed as vectors for cloning. Their construction incorporates mutations that make them simple to use and also greatly increases their safety for the biological containment of cloned recombinant DNA. Three of the Charon vector
Protocols
General protocols for growth of competent cells in microbial medium.
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
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