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N3129

Sigma-Aldrich

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt

phosphatase substrate

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Synonym(s):
p-Nitrophenyl phosphate bis(cyclohexylammonium) salt, PNPP
Linear Formula:
O2NC6H4OP(O)(O-)2 · 2C6H11NH3+
CAS Number:
52483-84-8
Molecular Weight:
417.44
Beilstein:
6553276
EC Number:
257-949-0
MDL number:
MFCD00013173
UNSPSC Code:
12352204
PubChem Substance ID:
24897623
NACRES:
NA.83

Quality Level

300

Assay

≥97% anhydrous basis

form

powder

solubility

water: 10 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

NC1CCCCC1.NC2CCCCC2.OP(O)(=O)Oc3ccc(cc3)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2

InChI key

KXNNUYCXODNFBD-UHFFFAOYSA-N

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Application

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

Biochem/physiol Actions

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(6), e21551-e21551 (2021-05-28)
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Bauerle WL, et al.
J. Am. Soc. Hort. Sci., 129(2), 182-187 (2004)
Leaf abscisic acid accumulation in response to substrate water content: Linking leaf gas exchange regulation with leaf abscisic acid concentration
Bauerle WL, et al.
J. Am. Soc. Hort. Sci., 131(2), 295-301 (2006)
Ziad Dahabreh et al.
World journal of stem cells, 6(4), 497-504 (2014-09-27)
To compare seven commercially available bone graft substitutes (BGS) in terms of these properties and without using any additional biological growth factors. Porcine osteoprogenitor cells were loaded on seven commercially available BGS and allowed to proliferate for one week followed
Bjarni Ásgeirsson et al.
Biochemistry and biophysics reports, 24, 100830-100830 (2020-10-27)
Para-nitrophenyl phosphate, the common substrate for alkaline phosphatase (AP), is available as a cyclohexylamine salt. Here, we report that cyclohexylamine is a non-competitive inhibitor of APs. Cyclohexylamine inhibited four different APs. Co-crystallization with the cold-active Vibrio AP (VAP) was performed

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