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N3013

Sigma-Aldrich

Nile Red

Powder

Synonym(s):

Nile Blue A Oxazone

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About This Item

Empirical Formula (Hill Notation):
C20H18N2O2
CAS Number:
7385-67-3
Molecular Weight:
318.37
Beilstein:
279110
EC Number:
230-966-0
MDL number:
MFCD00011639
UNSPSC Code:
12171500
PubChem Substance ID:
24897612
NACRES:
NA.47

product name

Nile Red, Technical grade

grade

technical grade

Quality Level

200

form

powder

composition

Carbon: 70.0-77.3%

Nitrogen: 7.5-8.8%

color

faint green to brown

mp

203-205 °C (lit.)

solubility

methanol: 1 mg/mL

λmax

553 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34

InChI

1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3

InChI key

VOFUROIFQGPCGE-UHFFFAOYSA-N

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General description

Nile Red is a lipophilic fluorescent dye that is commonly used in various scientific applications, particularly in lipid research. It has the ability to stain and visualize lipid droplets in cells and tissues.

Application

Nile red is widely employed in studies related to lipid metabolism, lipid droplet dynamics, lipid accumulation in cells or tissues, and lipid-based drug delivery systems.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Autophagy gene disruption reveals a non-vacuolar cell death pathway in Dictyostelium.
Kosta A
The Journal of Biological Chemistry, 279, 48404-48404 (2004)
D L Sackett et al.
Analytical biochemistry, 167(2), 228-234 (1987-12-01)
Nile red is an uncharged hydrophobic molecule whose fluorescence is strongly influenced by the polarity of its environment. It interacts with many, but not all, native proteins, including beta-lactoglobulin, kappa-casein, and albumin, with a wide range of spectral changes for
Zebrafish obesogenic test: a tool for screening molecules that target adiposity.
Tingaud-Sequeira A
Journal of Lipid Research, 52, 1765-1765 (2011)
Jiyoon Kim et al.
Nature communications, 11(1), 1418-1418 (2020-03-19)
The Golgi apparatus plays a central role in the intracellular transport of macromolecules. However, molecular mechanisms of Golgi-mediated lipid transport remain poorly understood. Here, we show that genetic inactivation of the Golgi-resident protein GRASP55 in mice reduces whole-body fat mass
Glycine N-methyltransferase deficiency affects Niemann-Pick type C2 protein stability and regulates hepatic cholesterol homeostasis.
Liao YJ
Molecular Medicine, 18, 412-412 (2012)
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