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N2889

Sigma-Aldrich

Neutral Red Solution (0.33%)

3.3 g/L in DPBS, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Neutral Red solution, 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Toluylene red

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About This Item

Empirical Formula (Hill Notation):
C15H17ClN4
CAS Number:
Molecular Weight:
288.78
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

product line

BioReagent

form

solution

concentration

3.3 g/L in DPBS

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

SMILES string

[Cl-].Cc1cc2N=C3C=C\C(C=C3Nc2cc1N)=[N+](/C)C

InChI

1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H

InChI key

PGSADBUBUOPOJS-UHFFFAOYSA-N

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General description

Neutral Red is a cationic dye that is used for nuclear counterstaining in biological research. It also serves as a pH indicator in analytical laboratories. On decomposition, neutral red dye releases carbon monoxide (CO), carbon dioxide (CO2), nitrogen oxides, and hydrogen chlorides. Therefore, this dye is considered toxic and is recommended to remove before discharge into receiving streams.

Application

Neutral Red Solution (0.33%) has been to stain viable cells and measure cell viability. It has also been used as a component of culture medium for enzymatic digestion of ovarian medulla tissue to extract follicles.

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WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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