N2787
Monoclonal Anti-Neurofilament 160 antibody produced in mouse
~2 mg/mL, clone RMO44, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Anti-Neurofilament Medium Chain
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About This Item
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RMO44, monoclonal
form
buffered aqueous solution
species reactivity
human, mouse, rat, zebrafish
concentration
~2 mg/mL
technique(s)
immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using extract of SHSy5y human neuroblastoma cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NEFM(4741)
mouse ... Nefm(18040)
rat ... Nefm(24588)
Related Categories
General description
Monoclonal Anti- Neurofilament 160 (mouse IgG1 isotype) is derived from the hybridoma RMO44 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified mid-sized rat neurofilament (NF-M) subunit. Neurofilaments are one of the five major groups of intermediate filaments (Ifs) and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kDa, 160 kDa and 200 kDa.
Neurofilament 160 (NEFM) is one among the most abundant filaments in post-mitotic neurons.
Immunogen
purified mid-sized rat neurofilament (NF-M) subunit.
Application
Monoclonal Anti-Neurofilament 160 antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- western blot analysis
- immunohistochemistry
- antibody labelling
Biochem/physiol Actions
Neurofilament 160 (NEFM) associates with the dopamine receptor.
Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals. These molecules undergo post-translational modifications, which results in their heterogeneity including different levels of phosphorylation. The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing their interaction with cytoplasmic organelles.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Flash Point(C)
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Regulatory Information
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