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N2415

Nucleoside Phosphorylase bacterial

recombinant, expressed in E. coli, ≥10 units/mg protein

Synonym(s):

PNP, Purine nucleoside phosphorylase, Purine nucleoside:orthophosphate ribosyltransferase

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About This Item

CAS Number:
9030-21-1
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-857-3
MDL number:
MFCD00131739
EC Number:
2.4.2.1 (BRENDA, IUBMB)

Key Documents

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Product Name

Nucleoside Phosphorylase bacterial, recombinant, expressed in E. coli, ≥10 units/mg protein

SMILES string

[n]2(c3ncncc3nc2)[C@@H]1O[C@@H]([C@H]([C@H]1O)O)CO

InChI

1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1

InChI key

MRWXACSTFXYYMV-FDDDBJFASA-N

recombinant

expressed in E. coli

assay

≥80% protein basis (biuret)

form

lyophilized powder

specific activity

≥10 units/mg protein

sequence note

MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE

UniProt accession no.

P0ABP8

storage temp.

−20°C

Quality Level

200

Gene Information

Escherichia coli ... deoD(945654)

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Application

Bacterial nucleoside phosphorylase has been used in a study that identified and characterized two adenosine phosphorylase activities in Mycobacterium smegmatis. Bacterial nucleoside phosphorylase has also been used in a study to investigate the inhibitionof pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-substituted 2-deoxy-D-ribose-1-phosphate derivative.
Nucleoside Phosphorylase bacterial has been used in reaction buffer and buffer-exchanged to remove residual phosphate.
Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation.

Biochem/physiol Actions

Catalyzes the following reaction:purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
Nucleoside Phosphorylase/purine nucleoside phosphorylase (PNP), an enzyme participates in the purine salvage pathway. It also helps in the reversible cleavage of inosine to hypoxanthine and guanosine to guanine. PNP deficiency results in neurologic manifestations.

Other Notes

One unit will cause the phosphorolysis of 1.0 micromole of inosine to hypoxanthine andribose-1-phosphate per min at pH 7.4 at 25 deg C.

Preparation Note

This is a salt-free and animal component-free enzyme preparation. This enzyme should be solubilized in a 10mM KPO4, pH 7.4 solution.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000492355
0000492355
0000488017
0000488017
0000466004

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Irina V Varizhuk et al.
Current protocols, 2(1), e347-e347 (2022-01-21)
A simple and efficient method for the preparation of α-D-ribose 1-phosphate and 2-deoxy-α-D-ribose 1-phosphate, key intermediates in nucleoside metabolism and important starting compounds for the enzymatic synthesis of various modified nucleosides, has been proposed. It consists in near-irreversible enzymatic phosphorolysis
Primary T-cell immunodeficiencies
Clinical Immunology (Orlando, Fla.), 54(7), 489-508 (2019)
Conformational stability and catalytic activity of PTEN variants linked to cancers and autism spectrum disorders
Johnston S B and Raines R T
Biochemistry, 54(7), 1576-1582 (2015)
Patricia Calero et al.
Nature communications, 11(1), 5045-5045 (2020-10-09)
Fluorine is a key element in the synthesis of molecules broadly used in medicine, agriculture and materials. Addition of fluorine to organic structures represents a unique strategy for tuning molecular properties, yet this atom is rarely found in Nature and
Disorders of purines and pyrimidines
Handbook of Clinical Neurology, 827-838 (2014)
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