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About This Item
Empirical Formula (Hill Notation):
C16H23NO4
CAS Number:
Molecular Weight:
293.36
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
Beilstein/REAXYS Number:
1915230
Product Name
4-Nitrophenyl decanoate, lipase substrate
InChI
1S/C16H23NO4/c1-2-3-4-5-6-7-8-9-16(18)21-15-12-10-14(11-13-15)17(19)20/h10-13H,2-9H2,1H3
SMILES string
CCCCCCCCCC(=O)Oc1ccc(cc1)[N+]([O-])=O
InChI key
RRNKCSIEGPOIKI-UHFFFAOYSA-N
assay
≥98% (TLC)
form
powder
solubility
chloroform: soluble 100 mg/mL, colorless to faintly yellow
storage temp.
−20°C
Quality Level
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Application
4-Nitrophenyl decanoate has been used as a substrate for assessing the lipase activity spectrophotometrically. It has also been used as a substrate for estimating the carboxylesterases activity.
4-Nitrophenyl decanoate has been used as a substrate to assess the carboxylesterase production by recombinant Penicillium griseoroseum T55 strain. It has also been used for general esterase activity assay of the hepatopancreas extract.
Biochem/physiol Actions
4-Nitrophenyl decanoate is a substrate for lipase enzyme. The extracellular lipase has greater hydrolysis reaction velocity with 4-nitrophenyl decanoate when compared with other 4-nitropheny esters.
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Bees of the genus Osmia are being used in crop pollination at an increasing rate. However, a short life expectancy of adult individuals limits the feasibility of their use. Cocoons of the red mason bee, Osmia rufa L. (Hymenoptera: Megachilidae)
Janaina Marques de Almeida et al.
Scientific reports, 8(1), 10000-10000 (2018-07-04)
We determined the effect of the His-tag on the structure, activity, stability and immobilization of LipC12, a highly active lipase from a metagenomic library. We purified LipC12 with a N-terminal His-tag and then removed the tag using tobacco etch virus
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