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MTOXH1011

Sigma-Aldrich

Cryopreserved Primary Human Hepatocytes Neonatal

Synonym(s):

Primary Human Hepatocytes

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.81

biological source

human liver (hepatocytes)

form

liquid

usage

sufficient for 1 96-well plate(s) (or 24-well plate (plateable lots))
 Suspension assays (suspension lots suitable for suspension assays)

packaging

vial of 4 mil. cells

manufacturer/tradename

LifeNet Health®

growth mode

adherent or suspension depending on specific lot

description

≥70%, confluent monolayer on day 5 (plateable lots)

technique(s)

cell culture | mammalian: suitable
genotyping: suitable

application(s)

ADME/TOX

relevant disease(s)

non-alcoholic steatohepatitis (NASH); non-alcoholic/simple fatty liver (NAFL)

shipped in

liquid nitrogen

storage temp.

−196°C

General description

We provide high viability cryopreserved hepatocytes, sourced from LifeNet Health® that predictably represents in vivo conditions. Applications for primary hepatocytes include in vitro evaluation of metabolism, drug-drug interaction, drug transporter activity, and toxicity of drug candidates. There are many other applications that encompass cryopreserved hepatocytes.

Application

See technical bulletins for detailed protocols.

Click lot availability link to get a complete list of currently available hepatocyte lots from LifeNet Health®.

Once the lot number(s) of interest are determined enter the lot number into the “Certificate of Analysis” request search engine at the bottom of the page to obtain a pdf copy of the CoA. Note, lot number CoA upload is only available on the product number detail page corresponding with the lot number.

Features and Benefits

These cryopreserved hepatocytes are sourced from LifeNet Health®. All cryopreserved hepatocytes have post-thaw viabilities over 80%. These lots are also characterized for Phase I CYP450 isoform and Phase II drug-metabolizing enzyme activity. Cryopreserved hepatocytes can be used for a variety of applications including metabolic stability, clearance, metabolite ID, metabolite profiling, species comparison studies, and uptake transporter studies.

Each lot is fully characterized for viability/stability, cell yield, attachment efficiency, optimal seeding density, monolayer confluency, Phase I and Phase II drug metabolizing enzyme activities and cyp induction. We have a wide selection of lots that can be used for a variety of applications, including, but not limited to:

  • Drug metabolizing enzyme activity (stability, clearance, metabolite profiling and ID)
  • Time-dependent metabolism (plated assay for low-clearance compounds)
  • CYP450 Induction (mRNA and activity)
  • Plated drug transporter assays (uptake and efflux)
  • Toxicity assays
  • High content analysis
  • High content imaging
  • 3D culture

Packaging

This product will be shipped in a liquid nitrogen dry shipper supplied from Cryoport. Please remember to ship the container back to Cryoport once you have removed the materials. Additional Cryoport shipping charges may apply.

Please see the Cryoport website for more details on the dry shipper.

Legal Information

LifeNet Health is a registered trademark of LifeNet Health

required but not provided

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

新产品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
LHUM17010R
LHUM15103

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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