MTOX1300
Canine MDCKII Wild Type Cells
canine
Synonym(s):
MDCK II, MDCK-2, MDCKII-WT
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.81
Biological source:
canine
Product Name
Canine MDCKII Wild Type Cells,
biological source
canine
form
liquid
storage temp.
−196°C
Quality Level
Analysis Note
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.
Application
Canine MDCKII Wild Type Cells can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells.
The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. See technical bulletin for detailed protocols.
Features and Benefits
MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron.
General description
MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435).
Legal Information
Exhibit 1: ADME/Tox cell lines licenseMDCKII subclone was originally isolated by Daniel Louvard, Institut Curie, Paris France.
Preparation Note
Upon receiving a shipment of frozen canine MDCKII wild type cells it is important the end user gives the shipment attention without delay. To ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at –70 °C. Storage at –70 °C will result in loss of viability.
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
监管及禁止进口产品
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D Louvard
Proceedings of the National Academy of Sciences of the United States of America, 77(7), 4132-4136 (1980-07-01)
A dog kidney epithelial cell line (MDCK), grown in monolayer, displayed in vitro an asymmetric localization of surface proteins. Aminopeptidase [alpha-aminoacylpeptide hydrolase (microsomal), EC 3.4.11.2] was found only in the apical face whereas Na+, K+-ATPase (ATP phosphohydrolase, EC 3.6.1.3) was
G C Hansson et al.
The EMBO journal, 5(3), 483-489 (1986-03-01)
The glycosphingolipids (GSLs) of two sublines of Madin-Darby canine kidney (MDCK) cells, an epithelial cell line, were characterized by t.l.c., antibody overlay and mass spectrometry. The major characteristic which distinguishes the two MDCK cell strains is their trans-epithelial electrical resistance
Protocols
Discover an expert protocol for optimizing MDCK-II cell growth on the Millicell® 96-well cell culture assembly with the Millicell® ERS 3.0.
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