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MSST0011

SILuProt ALB Albumin human

recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled

Synonym(s):

Albumin, LC/MS internal standard for human albumin in, stable isotope-labeled human albumin

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About This Item

NACRES:
NA.12
UNSPSC Code:
23201100
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Product Name

SILuProt ALB Albumin human, recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled

biological source

human

recombinant

expressed in HEK 293 cells

tag

His tagged

assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

≥98% Heavy amino acids incorporation efficiency by MS

concentration

≥10 μg/mg (Bradford)

technique(s)

mass spectrometry (MS): suitable

suitability

suitable for mass spectrometry (standard)

UniProt accession no.

storage temp.

−20°C

Quality Level

Gene Information

human ... ALB(213)

Legal Information

SILu is a trademark of Sigma-Aldrich Co. LLC

Biochem/physiol Actions

Albumin is synthesized exclusively by the liver parenchymal cells at a rate of 14 g/day. While it is the most abundant plasma protein (>50%), increased urine albumin concentrations indicate bleedings in the lower urinary tract (ureter, bladder) or infections of the renal pelvis. A small abnormal albumin excretion is known as Microalbuminuria and serves as an indicator for temporary overload of the glomerular filtration (fever, excessive sports) or chronic injury of the glomeruli (diabetes).

General description

SILuProt ALB is a recombinant, stable isotope-labeled human albumin which incorporates [13C6, 15N4]-Arginine and [13C6, 15N2]-Lysine. Expressed in human 293 cells, it is designed to be used as an internal standard for bioanalysis of albumin in mass-spectrometry. SILuProt ALB is a monomer of 607 amino acids (including C-terminal polyhistidine and flag tags), with a molecular weight of ~69 kDa.

Other Notes

RRDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGLDYKDDDDKGHHHHHHHHGGQ

Physical form

Supplied as a lyophilized powder containing phosphate buffered saline.

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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