MSSAFE
Protease and Phosphatase Inhibitor Cocktail
MS-SAFE, powder, Protease inhibitors: serine, cysteine and aspartic proteases and metalloproteases. Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases.
Synonym(s):
Mass Spectrometry Safe Protease and Phosphatase Inhibitor Cocktail
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.77
General description
Mass spectrometry (MS) compatible protease inhibitor cocktail and phosphatase inhibitor cocktail, with broad specificity for the inhibition of:
- Serine, cysteine and aspartic proteases, and metalloproteases
- Tyrosine, serine/threonine, acid and alkaline phosphatases
Specificity
Protease inhibitors: serine, cysteine and aspartic proteases, and metalloproteases
Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases
Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases
Application
MS-SAFE Protease and Phosphatase Inhibitor has been used to measure ribosomal protein S6 kinase (S6K) activity.
Tested in mammalian cell lysates and liver tissue extracts. Designed for use in samples to be analyzed by mass spectrometry.
Features and Benefits
Comprehensive protease and phosphatase inhibitor cocktail for mass spectrometry analysis
Compatible with downstream sample processing such as His-tagged protein purification and phosphopeptide enrichment
Allows accurate measurement of protein activity and identification of phosphorylation sites.
Compatible with downstream sample processing such as His-tagged protein purification and phosphopeptide enrichment
Allows accurate measurement of protein activity and identification of phosphorylation sites.
Components
Protease inhibitors:
- Bestatin hydrochloride
- Leupeptin
- Phosphoramidon disodium salt
- Pepstatin A
- Elastatinal
- Aprotinin
- Nafamostat mesylate
- Antipain
- Okadaic acid
- Sodium fluoride
- Sodium orthovanadate
- Bromotetramisole oxalate
- β-lactose
- DL-leucine
Quantity
One vial makes 20 mL of 1× inhibitor cocktail working solution, using either water or extraction/lysis buffer. Alternatively, a 10× concentrated solution may be prepared by adding 2 mL of water or extraction/lysis buffer. This 10× solution may then be diluted 10-fold into extraction/lysis buffer as needed for a 1× working solution.
Physical form
Lyophilized powder that is water-soluble
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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S K Sreedharan et al.
The Biochemical journal, 316 ( Pt 3), 777-786 (1996-06-15)
1-trans-Epoxysuccinyl-L-leucylamido(4-guanidino)butane (E-64) was shown to inhibit beta-trypsin by a reversible competitive mechanism; this contrasts with the widely held view that E-64 is a class-specific inhibitor of the cysteine proteinases and reports in the literature that it does not inhibit a
Junguo Ma et al.
Chemosphere, 194, 773-783 (2017-12-19)
A previous study showed that microcystin-LR (MC-LR) exerted cytotoxicity and induced apoptosis in HepG2 cells. In the present study, we investigated whether oxidative stress-mediated p53/p21
Aberrant REDD1-mTORC1 responses to insulin in skeletal muscle from Type 2 diabetics.
Williamson, D.L., et al.
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 309, R855-R863 (2015)
Mark D Schuchard et al.
BioTechniques, 39(2), 239-247 (2005-08-25)
The inclusion of protease inhibitors in serum or plasma samples has been found to significantly impact the isoform profile of selected plasma proteins as seen on 2-dimensional electrophoresis (2-DE) gels. With the addition of a protease inhibitor cocktail, several human
Yumei Li et al.
Oncology letters, 14(6), 8035-8041 (2018-01-19)
Rhabdoid tumors, which tend to occur prior to the age of 2 years, are one of the most aggressive malignancies and have a poor prognosis due to the frequency of metastasis. Silibinin, a natural extract, has been approved as a
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Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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