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MSKT0004

Sigma-Aldrich

IA-MS Immunoaffinity Mass Spectrometry Kit - Protein A/G

96 Well Strip Plate with SOLu-Trypsin

Synonym(s):

Target protein enrichment and quantification from complex background by mass spectrometry.

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About This Item

UNSPSC Code:
41115859
NACRES:
NA.32

form

solid

suitability

suitable for mass spectrometry

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

The Protein A/G IA-MS Immunoaffinity Mass Spectrometry Kit is a 96-well platform enabling robust, high-throughput quantification of proteins in complex backgrounds by LC-MS/MS. Users provide their own capture antibody and internal standard (available separately, internal standards may be SIL peptides or preferably full length SIL protein) and the kit contains all other components needed for in-well enrichment and rapid trypsin digestion of samples. The plate format consists of 8-well strips allowing for partial runs, and avoids the high cost and potential LC column blockage associated with bead-based enrichment formats. The entire workflow can be performed in under 5 hours, resulting in digests ready for injection with no SPE or other cleanup required.

Application

Target protein enrichment and quantification from complex background by mass spectrometry.

Features and Benefits

  • Robust, high-throughput quantification of proteins in complex backgrounds by LC-MS/MS
  • Entire workflow including digest completed in under 5 hours, such that samples are injection ready at the end of the work day
  • 96-well plate format has potential for automation in a liquid-handling system
  • Bead-free system circumvents messy clean-up and eliminates risk of column clogging
  • Plate strips allow user to run as many or as few samples as desired

Packaging

Box with foam insert containing kit components.

Components

Protein A/G Coated Plates SAE0113-1EA
SOLu-Trypsin EMS0004-100UG
MS Denaturation Solution EMS00010-10ML
Rapid Trypsin Digestion Buffer EMS0009-30ML
Tris Buffered Saline with TWEEN® 20 (TBST)powder, pH 8.0 T9039
Tris Buffered Saline (TBS) powder, pH 8.0 T6664
EZ-Pierce™ plate seal Z721581-1EA

See datasheet for more information.

Storage and Stability

2 years when stored at 2-8 °C.

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Legal Information

EZ-Pierce is a trademark of Excel Scientific, Inc.
TWEEN is a registered trademark of Croda International PLC

Kit Components Only

Product No.
Description
  • MS Denaturation Solution
  • Rapid Trypsin Digestion Buffer

Kit Components Also Available Separately

Product No.
Description
SDS
  • Protein A/G Coated Plates
  • EMS0004SOLu-Trypsin, recombinant, expressed in Pichia pastoris, Proteomics Grade, liquidSDS
  • T9039Tris Buffered Saline, with Tween™ 20, pH 8.0, powderSDS
  • T6664Tris Buffered Saline, pH 8.0, powderSDS
  • Z721581EZ-Pierce™ films, Excel Scientific EZP-100, non-sterileSDS

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 3 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

75.2 °F

Flash Point(C)

24 °C

Regulatory Information

新产品

Certificates of Analysis (COA)

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