MRN70
GenElute™ mRNA Miniprep Kit
sufficient for 70 purifications
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Synonym(s):
GenElute™ mRNA Kit, Gen Elute
UNSPSC Code:
12352200
NACRES:
NA.52
Recommended Products
usage
sufficient for 70 purifications
Quality Level
technique(s)
RNA purification: suitable
storage temp.
15-25°C
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General description
Procedures such as cDNA synthesis, expression profiling and others require separation of mRNA from the vastly more abundant rRNA and tRNA. The GenElute mRNA kits provide convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues. For direct mRNA preparation, cells or tissues are disrupted with SDS/proteinase K digestion to release RNA and eliminate RNases. Both kit types use oligo dT30 covalently linked to 1 μm polystyrene beads to capture polyadenylated mRNA by hybridization. The polystyrene beads remain suspended during hybridization, eliminating the need for mixing or rocking, as is common for cellulose or magnetic particles. Polystyrene was also chosen because oligo(dT) polystyrene beads yield cleaner mRNA with fewer stringent washing steps than does the more commonly used oligo(dT) cellulose (2 or 3 wash steps versus 10 or more). With the GenElute kits, mRNA-bead complexes are washed on a microcentrifuge spin filter, and eluted into 10 mM Tris-HCL, pH 7.5. mRNA prepared with either kit is suitable for a variety of downstream applications such as Northern blotting, expression array or chip hybridizations and cDNA synthesis and library construction.
Application
GenElute™ mRNA Miniprep Kit has been used to purify RNA from total RNA and to isolate RNA.
The purified mRNA is ready for Northern analysis, reverse transcription and PCR, labeling for arrays, and other common applications.
Features and Benefits
- Quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein
- Performs well with large or small amounts of tissue or cells and many samples can be simultaneously extracted
- One of the most effective methods for isolating total RNA. Purifications can be completed in only one hour starting with fresh tissue or cells
Other Notes
For additional information, please see www.sigma-aldrich.com/mrna.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
WGK
WGK 3
Certificates of Analysis (COA)
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Marimuthu Kumaravel et al.
Scientific reports, 10(1), 4501-4501 (2020-03-13)
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Transcriptome-wide mapping of N(6)-methyladenosine by m(6)A-seq based on immunocapturing and massively parallel sequencing.
Dominissini D
Nature Protocols, 8(1), 176-189 (2013)
Miguel Angel Garcia-Campos et al.
Cell, 178(3), 731-747 (2019-07-02)
N6-methyladenosine (m6A) is the most abundant modification on mRNA and is implicated in critical roles in development, physiology, and disease. A major limitation has been the inability to quantify m6A stoichiometry and the lack of antibody-independent methodologies for interrogating m6A.
Stephen DiGiuseppe et al.
Virology, 458-459, 93-105 (2014-06-15)
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DiGiuseppe S, Bienkowska-Haba M, Hilbig L, et al.
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Articles
Understand how mRNA vaccines induce immunity. and read how synthetic mRNA is prepared for vaccine immunogens and other biopharmaceuticals. Find reagents for synthesis of mRNA.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
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