MCL1
Mammalian Cell Lysis Kit
Synonym(s):
Cell Lysis Reagent
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.56
usage
kit sufficient for 250 extractions (100 mm tissue culture plates)
Quality Level
technique(s)
immunoprecipitation (IP): suitable
shipped in
dry ice
storage temp.
−20°C
Application
The product contains all required reagents for the disintegration of mammalian cells and tissue for subsequent analysis of protein targets. Cellular proteins are stabilized during lysis and are suitable for immunoassays, immunoprecipitation, affinity purification and other functional assays.
Mammalian cell lysis kit was used to test the role of vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration.
Mammalian cell lysis kit was used to test the role of vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration.
Features and Benefits
- The kit can be used to prepare cell lysis buffer and other modified buffers
- The kit enables the researcher to change the buffer′s components to obtain maximum efficiency for a specific protein
Other Notes
The mammalian cell lysis kit is composed of solutions to be mixed and used for lysis of adherent cells, non-adherent cells, and tissues. It contains buffer, detergents, NaCl and protease inhibitor cocktail solutions.
Kit Components Also Available Separately
Product No.
Description
SDS
- P8340Protease inhibitor cocktail 2.5 mL
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Shintaro Nakao et al.
Investigative ophthalmology & visual science, 54(6), 3830-3836 (2013-04-13)
Inflammation and immune cells regulate choroidal neovascularization (CNV) and could become therapeutic targets in age-related macular degeneration (AMD). Lymphangiogenesis is a key component of various inflammatory diseases. Whether lymphangiogenesis and lymph node-mediated immunity are involved in the pathogenesis of AMD
Shintaro Nakao et al.
Blood, 117(3), 1081-1090 (2010-08-14)
Angio- and lymphangiogenesis are inherently related processes. However, how blood and lymphatic vessels regulate each other is unknown. This work introduces a novel mechanism explaining the temporal and spatial relation of blood and lymphatic vessels. Vascular endothelial growth factor-A (VEGF-A)
Shinsuke Yasuda et al.
Journal of immunology (Baltimore, Md. : 1950), 179(7), 4890-4900 (2007-09-20)
Dysregulation of Ras guanyl nucleotide-releasing protein 1 (RasGRP1) in mice results in a systemic lupus erythematosus (SLE)-like disorder. We therefore looked for defective isoforms and/or diminished levels of human RasGRP1 in a cohort of SLE patients. PBMCs were collected from
Shintaro Nakao et al.
The American journal of pathology, 178(4), 1913-1921 (2011-03-26)
Vascular adhesion protein-1 (VAP-1) contributes to inflammatory and angiogenic diseases, including cancer and age-related macular degeneration. It is expressed in blood vessels and contributes to inflammatory leukocyte recruitment. The cytokines IL-1β and vascular endothelial growth factor A (VEGF-A) modulate angiogenesis
Yang Wang et al.
Autophagy, 12(6), 949-962 (2016-04-29)
Hydroxychloroquine (HCQ) inhibits autophagy and therefore can sensitize some cancer cells to chemotherapy, but the high doses required limit its clinical use. Here we show that loading HCQ into liposomes (HCQ/Lip) decorated with a pH-sensitive TH-RGD targeting peptide (HCQ/Lip-TR) can
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