MBD5000
GenElute™ Microbiome DNA Purification Kit
High yield for PCR, sequencing and NGS
About This Item
Recommended Products
Quality Level
technique(s)
DNA purification: suitable (from stool )
shipped in
ambient
storage temp.
room temp
Related Categories
1 of 4
This Item | 150357 | 150356 | 150268 |
|---|---|---|---|
| separation technique hydrophilic interaction (HILIC), supercritical fluid (SFC), normal phase | separation technique hydrophilic interaction (HILIC), normal phase | separation technique hydrophilic interaction (HILIC), normal phase | separation technique hydrophilic interaction (HILIC), normal phase |
| particle size 5 μm | particle size 5 μm | particle size 5 μm | particle size 5 μm |
| matrix fully porous particle | matrix fully porous particle | matrix fully porous particle | matrix fully porous particle |
| product line Purospher® | product line Purospher® | product line Purospher® | product line Purospher® |
| pore size 120 Å pore size | pore size 120 Å pore size | pore size 120 Å pore size | pore size 120 Å pore size |
Application
The Microbiome DNA Purification Kit provides a convenient and rapid method to isolate high-molecular weight, high-quality and high yield microbial DNA from stool samples. The kit contains DNA-free reagents to assure that no external contamination is present. The kit uses a combination of chemical, mechanical and thermal lysis with a simple and rapid spin column procedure to purify the DNA. The purified DNA is high molecular weight, high quality and is fully compatible with downstream applications, such as PCR and NGS.
application note: Typical DNA recovery is 5–25μg from 0.2 g of human stool or 0.1 g of mouse stool.
Features and Benefits
- Accurate and reproducible results
- Convenient and rapid spin column procedure
- Highly efficient lysis: combination of chemical, mechanical and thermal methods
- High molecular weight, high quality and high yield DNA is obtained
- DNA free reagents to assure no external contamination
- Fully compatible with downstream applications such as PCR, sequencing and NGS
Components
.
- This product is comprised of the following:
- M1 Lysis Buffer MBD5001
- M2 Lysis Boost Buffer MBD5002
- M3 Cleaning Buffer MBD003
- M4 Binding Buffer MBD5004
- M5 Wash Buffer MBD005
- M6 Elution Buffer MBD006
- Columns MBD5007
- Collection Tubes MBD5008
- Lysis Beads MBD5009
See datasheet for more information
Legal Information
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
Supplementary Hazards
Storage Class Code
8A - Combustible corrosive hazardous materials
Regulatory Information
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