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MBD0007

Sigma-Aldrich

Inactivated Proteus mirabilis

Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml

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UNSPSC Code:
41105500
NACRES:
NA.51

Quality Level

200

form

liquid

concentration

>10^8 bacteria/ml

technique(s)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Standardization of sample analysis is currently neededin microbiome genomics research workflow. Lack of standardization can lead tobiases and errors in common processes during sample preparation and analysissuch as sample amplification, sequencing and bioinformatics analyses.1 Inactivated Proteus mirabilis can serve as standard for benchmarking theperformance along the workflow of microbiomics or meta-genomics analyses and asa tool to increase reproducibility and allow comparison of results obtained bydifferent labs.
Proteusmirabilis is a gram negative facultative anaerobicrod-shaped bacterium. Bacteria of the genus Proteus of the familyEnterobacteriaceae are opportunistic human pathogens responsible for wound andburn infections as well as skin, eye, ear, nose, throat, urinary tract, andgastrointestinal infections and bacteremias.2 The most common infection involving P. mirabilis occurs when the bacteria, which is a memberof the natural intestinal flora, moves to the urethra and urinary bladder causingurinary tract infection. The outer-membrane lipopolysaccharide (LPS) isconsidered an important virulence factor of Proteus.3 Arabski et al. suggest that theimmunological response against P. mirabilis LPS might play a role in rheumatoidarthritis.4 A possible correlation between the abundanceof P. mirabilis in the intestine and obesity was suggestedrecently.5


Read here how to use our standards to ensure data integrity for your microbiome research.

Application

Inactivated bacteria are provided at >10^8 bacteria/ml concentration in TE buffer pH 8.0. It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS

Features and Benefits

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Physical form

Liquid -The inactivated bacteria is provided at >10^8 bacteria/ml in TE buffer pH 8.0

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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