MAK539
DNA Assay Kit
sufficient for 250 fluorometric tests
Sign Into View Organizational & Contract Pricing
All Photos(1)
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
General description
DNA quantitation is a common practice in molecular biology. Very often DNA is available in minute quantities and the traditional UV 260 nm absorbance method requires microgram quantities for reliable results. Accurate determination of DNA concentration, especially when DNA is present at low concentrations, is crucial for reproducible results in sequencing, cloning, transfection and DNA labeling.
Features and Benefits
- Linear detection range 2 ng to 40 ng (100 – 2,000 ng/mL) calf thymus DNA.
- Compatible with high-throughput handling systems.
- Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01% SDS) and common DNA extraction buffer do not interfere in the assay.
Suitability
The kit is suitable for quantitative determination of DNA in plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices.
Principle
The DNA Assay Kit is designed to accurately measure nanogram quantities of plasmid DNA, cDNA, DNA following polymerase chain reaction (PCR), and DNA eluted from gels. The improved method utilizes Hoechst dye that binds specifically with double-stranded DNA. The fluorescence intensity, measured at λEx = 350 nm/λEm = 450 nm, is directly proportional to the DNA concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
WGK
WGK 1
Regulatory Information
新产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service