MAK524
Isocitrate Assay Kit
sufficient for 100 colorimetric tests
Sign Into View Organizational & Contract Pricing
All Photos(1)
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
Quality Level
application(s)
food and beverages
detection method
colorimetric
relevant disease(s)
cancer
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
Isocitrate (isocitric acid) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.
Features and Benefits
- Linear detection range: 20 to 5000 µM
- Compatible with high-throughput handling systems.
Suitability
The kit is suitable for isocitrate determination in food, beverages, and biological samples (e.g. cell lysate, tissue homogenate, serum, etc.).
Principle
The Isocitrate Assay Kit measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the isocitrate concentration.
WGK
WGK 2
Regulatory Information
含少量动物源组分生物产品
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service