MAK500
Alanine Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
Assay Kit for Alanine Detection
UNSPSC Code:
12352204
NACRES:
NA.84
Recommended Products
Quality Level
application(s)
food and beverages
pharmaceutical
detection method
colorimetric
fluorometric
relevant disease(s)
obesity; cardiovascular diseases; cancer
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
Alanine, a nonessential amino acid, is utilized in the glucose-alanine cycle between tissues and the liver. In tissues that metabolize amino acids, amino groups are collected as glutamate by transamination. The amine group is then transferred by alanine transaminase (ALT) from glutamate to pyruvate to form alanine and α-ketoglutarate. The alanine generated is transported to the liver where a reverse ALT reaction occurs and pyruvate is regenerated. Pyruvate is converted through gluconeogenesis to glucose which can then be recirculated to the tissues. Alanine concentration may have some correlation with high blood pressure, energy intake, cholesterol levels and body mass index.
Features and Benefits
- Linear detection range: 1 to 200 µM alanine for the colorimetric assay and 0.4 to 20 µM for the fluorometric assay.
Suitability
The kit is suitable for determining alanine levels in plasma, serum, urine, tissue and culture media, as well as for studying the effects of drugs on alanine metabolism.
Principle
In the Alanine Assay Kit, alanine is converted into pyruvate which can then be directly measured. The color intensity of the reaction product at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the alanine concentration in the sample.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
WGK
WGK 2
Regulatory Information
新产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service