MAK450

Glucose Dehydrogenase Assay Kit

Name: Glucose Dehydrogenase Assay Kit
Brand: SIGMA

Sufficient for 100 colorimetric tests

Synonym(s):

GDH Activity Assay Kit

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About This Item

NACRES:

NA.84

UNSPSC Code:

12161503

Key Documents

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Product Name

Glucose Dehydrogenase Assay Kit, Sufficient for 100 colorimetric tests

storage condition

(No metal containers. Keep container tightly closed in a dry and well -ventilated place. Keep away from heat and sources of ignition.)

input

tissue
cell culture medium
tissue
plasma

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; hematological disorder

storage temp.

−20°C

Quality Level

100

Related Categories

Biochemicals

Metabolism Assay Kits

Application

The Glucose Dehydrogenase Assay Kit may be used for:

Metabolism Research
Drug Discovery

The kit is suitable for the quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism in biological samples (e.g. plasma, serum, tissue and culture media).

Biochem/physiol Actions

The Glucose Dehydrogenase Activity Assay Kit is a non-radioactive, colorimetric GDH assay. It is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A₅₆₅nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.

Features and Benefits

Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose dehydrogenase levels across a broad range from 0.5 to 200 U/L, providing both convenience and sensitivity.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

General description

Glucose Dehydrogenase (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.