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MAK450

Sigma-Aldrich

Glucose Dehydrogenase Assay Kit

Sufficient for 100 colorimetric tests

Synonym(s):

GDH Activity Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

100

storage condition

(No metal containers. Keep container tightly closed in a dry and well -ventilated place. Keep away from heat and sources of ignition.)

input

tissue
cell culture medium
tissue
plasma

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; hematological disorder

storage temp.

−20°C

Related Categories

General description

Glucose Dehydrogenase (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.

Application

The Glucose Dehydrogenase Assay Kit may be used for:
  • Metabolism Research
  • Drug Discovery

Features and Benefits

  • Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose dehydrogenase levels across a broad range from 0.5 to 200 U/L, providing both convenience and sensitivity.
  • Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Suitability

The kit is suitable for the quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism in biological samples (e.g. plasma, serum, tissue and culture media).

Principle

The Glucose Dehydrogenase Activity Assay Kit is a non-radioactive, colorimetric GDH assay. It is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Signal Word

Danger

Hazard Statements

H225,H290,H315,H319,H336

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

Regulatory Information

常规特殊物品
含少量动物源组分生物产品

Certificates of Analysis (COA)

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