MAK447
Alkaline Phosphatase Assay Kit
Sufficient for 250 colorimetric tests
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Synonym(s):
ALP Activity Assay Kit, Alkaline Phosphatase Test Kit
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
Quality Level
application(s)
pharmaceutical
detection method
colorimetric
relevant disease(s)
osteoporosis; gastrointestinal diseases; orthopedic diseases; cancer
storage temp.
−20°C
Related Categories
General description
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis.
Application
The Alkalnine Phosphatase Assay Kit may be used for:
- Cancer Research
- Gastroenterology Disease Research
- Osteoporosis Research
Features and Benefits
Enhanced Detection Range: Detect ALP levels accurately across a wide range, from 2 U/L to 800 U/L, using only 5 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a short 5 minute assay reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a short 5 minute assay reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Suitability
The kit is suitable for the quantitative determination of alkaline phosphatase (ALP) activity in serum, plasma etc.
Principle
The Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at A405 nm). The rate of the reaction is directly proportional to the enzyme activity.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
Target Organs
Nervous system,Kidney,Liver,Skin
WGK
WGK 3
Regulatory Information
新产品
Certificates of Analysis (COA)
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