MAK441
Kinase Assay Kit
Sufficient for 400 fluorometric tests
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Related Categories
General description
Kinases, alternatively referred to as phosphotransferases, form an extensive family of enzymes responsible for transferring phosphate groups from the high-energy donor molecule ATP to their designated substrates. This family of enzymes is renowned for its role in regulating a myriad of cellular processes, with the protein kinases constituting the largest subgroup. In humans, 518 distinct kinases have been identified, and a substantial portion of human proteins, up to 30%, undergo modification by these kinases. The considerable diversity and pivotal role of kinases in cellular signaling position them as promising targets for drug development, biochemical and biomedical research.
Application
The Kinase Assay Kit may be used for:
- Cancer Research
- Cell Signaling Research
- Drug Discovery
Features and Benefits
- High Sensitivity and Accuracy: Achieve quantification of kinase activity, detecting as low as 0.01 U/L with high sensitivity and accuracy.
- Streamlined Procedure: Effortlessly conduct the assay with a simplified process, adding just a single working reagent. Complete the assay in a quick 10-minute reaction, optimizing efficiency and saving valuable time.
- High-Throughput Inhibitor Screening: Screen for kinase inhibitors effectively with a minimal 5 μL test compound requirement, enhancing efficiency in high-throughput settings.
Suitability
The kit is suitable for the determination of protein kinase activity and for high-throughput screening of kinase inhibitors.
Principle
The Kinase Assay Kit provides a simple and rapid method for assaying kinase activity and high-throughput screening for kinase inhibitors. This homogeneous microplate-based assay involves incubating the kinase with a single working reagent, in which ADP is enzymatically converted to ATP and pyruvate, which is quantified using a fluorometric (Ex = λ530 nm/λEm = 590 nm) assay method.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Storage Class Code
10 - Combustible liquids
Regulatory Information
新产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service