Inorganic Polyphosphate Assay Kit (Fluorometric)

Inorganic polyphosphate (Poly P) is a linear polymer of hundreds of orthophosphate (Pi) residues linked by high-energy, phosphoanhydride bonds. Poly P is ubiquitous and can be found in all living organisms from bacteria to mammals. Poly P has many important cellular functions. In microorganisms, Poly P plays an important role in virulence, biofilm formation, motility, quorum sensing, stress response and survival during nutrient deficiency. In mammals, it is important for blood coagulation, calcium precipitation, immune response, apoptosis, signal transduction and mitochondrial metabolism. Poly P also plays an important role in cancer cell proliferation. The traditional assay for Poly P quantification uses radioisotope methods, which are not convenient to perform.

The kit is suitable for the determination of Poly P in various biological samples such as cell and tissue (kidney, brain, muscle etc.) lysate.

The assay uses a fluorescent dye that forms a complex with Poly P present in the sample and the fluorescent complex is measured at λ_Ex = 415 nm/λ_Em = 550 nm. The fluorescent signal is proportional to the Poly P concentration in the samples.

Inorganic Polyphosphate Assay Kit:

• Rapid and sensitive assay, detecting as low as as low as 50 pmole under the assay conditions.