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MAK428

Sigma-Aldrich

Glycolic Acid Assay Kit (Fluorometric)

sufficient for 100 fluorometric tests

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About This Item

application(s)

cosmetics
food and beverages

detection method

fluorometric

storage temp.

−20°C

General description

Glycolic Acid (GA), also known as Hydroxyl Acetic Acid is the smallest alpha hydroxy acid. Although it is used in several skin care products intended for dermal application in small doses, its acute dose can lead to skin and eye irritation. It is nephrotoxic if swallowed and its toxicity is due to its conversion to oxalic acid, which then precipitates with calcium to form crystals leading to renal tissue injury. GA is also a major downstream metabolite of ethylene glycol metabolism, and a concentration higher than 8 mM in serum indicates the need for hemodialysis. Normally serum GA levels lie in the very low micromolar range (roughly 2 μM).

Features and Benefits

Glycolic Acid Assay Kit:
  • One step plate based assay.
  • The kit can quantify as low as 30 pmol of GA.

Suitability

The kit is suitable for the measurement of glycolic acid in serum, plasma and urine

Principle

The Glycolic Acid Assay Kit is a robust, one step plate based assay for the measurement of GA in serum, urine and plasma samples. The GA Detection Reagent metabolizes GA leading to the formation of an intermediate. The intermediate in the presence of GA enzyme mix then converts the non-fluorescent GA probe to a fluorescent product, which is measured at λEx = 535 nm/λEm = 587 nm.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 3

Regulatory Information

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