MAK427
Proline Assay Kit (Fluorometric)
sufficient for 100 fluorometric tests
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About This Item
Recommended Products
application(s)
cosmetics
food and beverages
detection method
fluorometric
relevant disease(s)
immunological diseases
storage temp.
−20°C
General description
Proline is a proteogenic amino acid, which plays an important role in protein folding. In humans, proline can be synthesized from glutamate and arginine. Proline is one of the conditionally essential amino acids in humans. Proline is also produced by honeybees as they process nectar into honey. Thus, its content in honey is often used as an indicator of honey ripeness and sugar adulteration. For mature honey, 180 mg-proline/kg of honey is used as an international standard. Proline contents are measured for its biological importance in plants where it also serves as a stress marker. Unstressed plants contain approximately 0.5 μmol proline per gram of plant tissue while stressed plants can range up to 100 times more proline than the unstressed ones.
Features and Benefits
Proline Assay Kit:
- Compatible with high-throughput handling systems.
- Sensitive, detecting as low as 5 pmol of proline per well.
Suitability
The kit is suitable for the measurement of proline in biological samples such as serum, honey and mammalian and plant tissue. The kit can also be used in the analysis of honey maturation and in assaying proline concentration as a stress factor in plants.
Principle
In this assay, proline is converted to an intermediate, which will further react with a probe to produce a strong fluorometric signal (λEx = 535 nm/λEm = 587nm).
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
Regulatory Information
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